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Transplantation of GDNF-producing adult neural stem cells for cervical spondylosis model

产生GDNF的成体神经干细胞移植用于颈椎病模型

基本信息

批准号：
17591514
负责人：
MIYOSHI Yasuyuki
金额：
$ 2.35万
依托单位：
Okayama University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17591514/
关键词：
GDNF adult neural stem cell dial cells Neuron inflammatory cells agarose BBB scores グリア頚髄症アドノウイルスグリオーシス

项目摘要

The appropriate model of cervical spondylosis has not been established, although the number of patients with cervical spondylosis was increasing. We attempted to make the model to put agarose beads extradurally. On making cervical spondylosis model of rat, slipped agarose beads might be the major problem. In order to oppress surely spine for a long lime, slipped agarose beads were prevented by Gore Tex sheet and wide laminotomy were also performed for the lateral compression. Fibrin glue was applied to the agarose beads placed epidurally with firm attachment of Gore Tex sheet. After using this method, no slipped agarose beads were recognized at euthanasia. In this cervical spondylosis model of rats, BBB scores were significantly deteriorated and histologically spinal damages were larger than that of the control rats. By the transplantation of GDNF-secreling neural stem cells, BBB scores were ameliorated, although the improvement was not significant Transplanted GDNF-secreting cells survived for at least 1 month and secreted GDNF. GDNF-concentration near the transplanted site was amplified about 5 times, compared to the normal tissue. Histologically, transplantation could not reduce damaged area significantly and inflammatory cells increased around the transplanted site. Prevention of enhanced inflammatory cells might be a key to improve the cell therapy far cervical spondylosis model of rats.

颈椎病的发病率逐年上升，但至今尚未建立合适的颈椎病模型。我们尝试将琼脂糖珠置于硬膜外。在制作大鼠颈椎病模型时，琼脂糖珠的滑落可能是主要的问题。为确保脊柱长期受压，采用戈尔Tex片防止琼脂糖珠滑落，并行椎板大切开侧方加压。将柔性胶施加到琼脂糖珠上，琼脂糖珠以牢固的方式放置在戈尔特克斯片上。使用该方法后，在安乐死时未识别出滑动的琼脂糖珠。在该颈椎病模型中，大鼠的BBB评分明显恶化，脊髓组织学损害比对照组大。通过移植分泌GDNF的神经干细胞，BBB评分得到改善，尽管改善不显著。移植的分泌GDNF的细胞存活至少1个月并分泌GDNF。与正常组织相比，移植部位附近的GDNF浓度扩增约5倍。组织学上，移植不能明显缩小损伤面积，移植部位周围炎性细胞增多。预防炎症细胞的增加可能是提高细胞治疗大鼠颈椎病模型疗效的关键。