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Astudy of arthritic mechanism of the temporomandibular joint disorders

颞下颌关节疾病的关节炎机制研究

基本信息

批准号：
17592069
负责人：
TAKATSUKA Shigeyuki
金额：
$ 2.3万
依托单位：
Kanazawa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

项目摘要

1. Experimental study of temporomandibular joint arthritis in the rabbitsProteolytic activities of the temporomandibular arthritis have been investigated using a rabbit model of temporomandibular disorders. Histopathologic examination has been achieved on the arthritis caused by an artificial dislocation of the articular disc. Throughout the examination period, histoimmunochemical staining showed expression of proteolytic enzymes such as matrix metalloproteinases (MMPs) and aggrecanase(ADAMTS). Especially in the critical destructive period, specimens showed higher incidence of enzymatic expression. Expression of type I and H collagen stain were also noticed in the cartilage layer of the mandibular condyle. Distribution of these components differed according to the destruction periods.2. Analyses of the clinically harvested materialsHistopatholigic and biochemistric examination have been carried out using human materials of synovial fluid and biopsy tissue from the upper joint compartment of the temporomandibular joints, under informed consent. In patients with prominent arthritic changes on Milk showed higher incidence of MMP5 and ADAMTS. During cartilage matrix destruction, ADAMTS first acts on proteoglycan breakdown, then MMPs act on proteoglycan and collagen breakdown. These enzymatic expressions have been noticed in RA patients in the field of Orthopedic Surgery. Conversely in the present study, high incidence of both enzymatic activities has been noticed in the temporomandibular joints. From the results, arthritis may be mild in the temporomandibular joints. Harvested adhesion tissue via arthroscopic surgery showed a number of fibro-blastic cells. Specimens from advanced stage of arthritis showed ossification or cartilaginous degeneration. These lesions are frequently showed in preoperative MRI images.

1.本实验采用兔颞下颌关节紊乱病模型，研究了颞下颌关节炎的蛋白水解活性。对人工关节盘脱位引起的关节炎进行了组织学检查。在整个检查期间，组织免疫化学染色显示蛋白水解酶如基质金属蛋白酶（MMPs）和聚集蛋白聚糖酶（ADAMTS）的表达。特别是在临界破坏期，标本表现出较高的酶表达率。髁状突软骨层可见I型和H型胶原染色。这些组分的分布随破坏时期的不同而不同.临床采集材料的分析在知情同意的情况下，使用来自颞下颌关节上关节室的滑液和活检组织的人体材料进行了组织化学和生物化学检查。在有明显关节炎改变的患者中，牛奶显示MMP5和ADAMTS的发生率较高。在软骨基质破坏过程中，ADAMTS首先作用于蛋白多糖的分解，然后MMPs作用于蛋白多糖和胶原的分解。这些酶的表达已在骨科手术领域的RA患者中被注意到。相反，在本研究中，两种酶活性的高发病率已注意到在颞下颌关节。从结果来看，关节炎可能是轻微的颞下颌关节。通过关节镜手术采集的粘连组织显示出大量成纤维细胞。晚期关节炎的标本表现为骨化或软骨变性。这些病变常在术前MRI图像中显示。