Characterization of psychrophilic enzymes in ice algal species with a sequencing protocol of DNA from an individual algal colony

利用单个藻群 DNA 测序方案表征冰藻物种中的嗜冷酶

• 批准号: 17613005
• 负责人: SUZUKI Yoshihiro
• 金额: $ 2.35万
• 依托单位: Kanagawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17613005/
• 关键词: Ice lagae; rbcL; rbcS; RuBisCO; Cold Adaptation; psychrophilic

Ice algal species can grow just at the freezing point of seawater at below 0℃ and are often fond in a colored ice in the polar sea. These ice algal species must maintain their photosynthetic activities to grow at the low temperatures. We found these ice algal communities, which were constructed by many diatom species, at Saromako Lagoon, Hokkaido Japan. They also could grow and maintain their photosynthesis at the low temperatures. To estimate the maintenance mechanisms of their photosynthesis, we focused on the key enzyme of photosynthesis, ribulose 1, 5-bisphosphate carboxylase/oxygenase (EC. 4.1.1.39, RuBisCO here after) and determined the primary structures. As most of the ice algal species can not be incubated for a long time, it is hard to get an enough biomass for extraction of DNA. Isolation and amplification method of DNA from a single colony of algae (Iwatani, et al. 2002) were applied for this study and successfully determined the DNA sequences of large and small subunit of some ice algal RuBisCO.Although characteristic structures were not found in the small subunits, commonly different structures from those of the temperate diatom species were found in the large subunits of ice lagal RuBisCO, which constructed a loop between the a-helixes of #16 and #17. These structures of large subunits of ice algal RuBisCO could keep the flexibilities of these enzymes at low temperature

冰藻在海水冰点0℃以下即可生长，在极地海洋中常生长于彩色冰中。这些冰藻必须保持其光合作用活性，才能在低温下生长。我们在日本北海道的Saromako泻湖发现了这些冰藻群落，它们由许多硅藻物种组成。在低温条件下，它们也能生长并维持光合作用。为了探讨其光合作用的维持机制，我们对光合作用的关键酶核酮糖1，5-二磷酸羧化酶/加氧酶（EC）进行了研究。4.1.1.39，下文中的RuBisCO），并确定了一级结构。由于冰藻大多不能长时间培养，很难获得足够的生物量来提取DNA。单菌落藻类DNA的提取和扩增方法（Iwatani，et al.2002）成功地测定了一些冰藻RuBisCO的大小亚基的DNA序列，虽然在小亚基中没有发现特征结构，但在冰藻RuBisCO的大亚基中发现了与温带硅藻不同的结构，它在16号和17号的α螺旋之间构成了一个环。冰藻RuBisCO的这些大亚基结构可以保持这些酶在低温下的灵活性

项目成果

Phoyosynthetic responses of Japanese lily to light conditions on the understory of a temperate deciduous forest

日本百合对温带落叶林林下光照条件的光合成反应

• 发表时间: 2007
• 作者: M.Kono;Y.Suzuki
• 通讯作者: Y.Suzuki

Energy from the Sun:14th International Congress on Photosynthesis

来自太阳的能量：第14届国际光合作用大会

• 发表时间: 2008
• 作者: John F.Allen;Elisabeth Gantt;John H.Golbeck編
• 通讯作者: John H.Golbeck編

Photosynthesis. Energy from the Sun : 14th

光合作用。

• 发表时间: 2008
• 期刊: International Congress on Photosynthesis 2008
• 作者: John F. Allen、Elisabeth Gantt、John H. Golbeck;Ed. Springer
• 通讯作者: Ed. Springer

Photosynthetic responses of Japanese lily to fluctuated light condition on the understory of a temperate deciduous forest in early spring.

早春温带落叶林林下日本百合对波动光照条件的光合反应。

• 发表时间: 2007
• 期刊: Proc. 14th Intern. Photosyn. Cong. 24
• 作者: M.Kono;A. Dedachi;Y. Suzuki
• 通讯作者: Y. Suzuki

Phoyosynthetic responses of Japanese lily to lighi conditions on the understory of a temperate deciduous forest.

日本百合对温带落叶林林下林下条件的光合成反应。

• 发表时间: 2007
• 作者: M. Kono;Y. Suzuki.
• 通讯作者: Y. Suzuki.