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EVALUATION OF FOODS ALLERGY USING A VOLUNTARY PHYSICAL ACTIVITY IN MICE, AND ITS APPLICATION AS EVALUATION OF PROCESSED FOODS

利用小鼠自愿体力活动评估食物过敏及其在加工食品评估中的应用

基本信息

批准号：
18500634
负责人：
YANO Hiromi
金额：
$ 2.51万
依托单位：
Kawasaki University of Medical Welfare
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

项目摘要

In this study, we evaluated whether the voluntary physical activity, i.e., the revolution of a running wheel, is a novel and unique indication for the evaluation of the active systemic anaphylaxis in a mouse model or not. B10.A mice were immunized with egg lysozyme (LYZ) to induce specific IgE response, subsequently received an i.v. injection of the antigen, and then subjected to the body temperature measurement followed by the counting of the running wheel revolution. The injection with antigen inhibited in both the rectal temperature and the voluntary activity significantly reduced. The injection with histamine or leukotriene, which have been thought the main chemical mediators of anaphylaxis, significantly reduced the rectal temperature, but did not affect the wheel-running activity. In fact, although a high correlation between the total wheel-revolution and the lowering of body temperature caused by the antigen injection was observed (r=0.812, p<0.01), neither histamine nor leukotriene treated mice was shown the significant correlation.Furthermore, we determined the role of mast cells, which are the main cells of histamine and leukotriene production, on anaphylaxis in mice using mast cells deficient mice. As the results, although mast cells deficient mice also induced anaphylaxis after antigen re-exposure, the anaphylactic reactions were prevented by platelet-activating factor (PAF) receptor antagonist.These results suggest that the voluntary physical activity is a novel indication for the analyses of hypersensitivity reactions caused by LYZ re-exposure in the sensitized mice. In addition the reduction of physical activity in the sensitized mice might not be caused by must cells induced histamine and leukotriene.

在这项研究中，我们评估了自愿体力活动（即跑步轮的旋转）是否是评估小鼠模型中主动全身性过敏反应的新颖且独特的指标。 B10.A 小鼠用卵溶菌酶 (LYZ) 进行免疫以诱导特异性 IgE 反应，随后接受静脉注射。注射抗原，测量体温，计数跑轮转数。注射抗原受到抑制后，直肠温度和自主活动均显着降低。注射组胺或白三烯被认为是过敏反应的主要化学介质，可显着降低直肠温度，但不影响跑轮活动。事实上，虽然观察到总轮转数与抗原注射引起的体温降低之间存在高度相关性（r=0.812，p<0.01），但组胺或白三烯治疗的小鼠均未显示出显着相关性。此外，我们利用肥大细胞确定了肥大细胞（组胺和白三烯产生的主要细胞）对小鼠过敏反应的作用。细胞缺陷小鼠。结果，虽然肥大细胞缺陷小鼠在再次暴露于抗原后也会诱发过敏反应，但血小板活化因子（PAF）受体拮抗剂可以阻止过敏反应。这些结果表明，自愿体力活动是分析致敏小鼠中LYZ再次暴露引起的过敏反应的新指标。此外，致敏小鼠体力活动的减少可能不是由必须细胞诱导的组胺和白三烯引起的。