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Integrated and Fictionalized Nanoparticles in 3-dimentional nanospase

三维纳米空间中的集成和虚构纳米粒子

基本信息

批准号：
18510099
负责人：
TOMINAGA Masato
金额：
$ 2.64万
依托单位：
Kumamoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

项目摘要

Carbon nanotubes(CNTs)was synthesized on a platinum plate(99.98 %, 20×20×1 mm)by the chemical vapor deposition(CVD)method using iron nanoparticles derived from ferritin molecules. The synthesized CNTs were characterized by Raman spectroscopy and TEM. Two Raman shift peaks corresponding to G-(ca. 1580 cm^<-1>)and D-bands(ca. 1350 cm^<-1>)were observed on the platinum electrode surface after the synthesis of the CNTs, which indicated that CNTs were present on the electrode surface. The diameters of individual CNTs were evaluated to be ca. 5 to 10 nm, which could be classified as multi-walled CNTs(MWCNTs).The MWCNTs synthesized on the Pt plate (MWCNTs/Pt)electrode were immediately immersed into solutions of glucose oxidase (GOX)to immobilize these enzymes onto the MWCNTs/Pt electrode surfaces. After the GOX was immobilized onto the MWCNTs/Pt electrode, a well-defined catalytic oxidation current was increased from ca. -0.45 V (vs. Ag/AgCl/sat'd KCl), which was close to the redox potential of flavin adenine dinucleotide (FAD)as a prosthetic group of GOX under physiological pH values. Also, fructose dehydrogenase (FDH)immobilized MWCNTs/Pt electrode showed a well-defined catalytic oxidation current based on FDH was observed from ca. -0.15 V (vs. Ag/AgCl/sat'd KCl), which was close to the redox potential of heme c as a prosthetic group of FDH. From an analysis of a plot of the catalytic current vs. substrate, the calibration range for the fructose concentration was up to ca. 40μmol dm^<-3>, and the apparent Michaelis-Menten constant was evaluated to be 11 (±)mmol dm^<-3>Further investigations concerning both the interactions between enzymes and MWCNTs and direct heterogeneous electron transfer reactions are underway to develop sensor applications and glucose-oxygen biofuel cells.

采用化学气相沉积（CVD）法，在铂片（99.98%，20×20×1 mm）上，利用铁蛋白分子衍生的铁纳米颗粒合成了碳纳米管（CNTs）。用拉曼光谱和透射电镜对合成的碳纳米管进行了表征。对应于G-（ca. 1580 cm^<-1>）和D-波段（ca.在<-1>CNT合成后，在铂电极表面上观察到约1350 cm-2），这表明CNT存在于电极表面上。单个CNT的直径被评估为约100 μ m。将在Pt电极上合成的多壁碳纳米管（MWCNTs/Pt）直接浸入葡萄糖氧化酶（GOX）溶液中，使葡萄糖氧化酶固定在MWCNTs/Pt电极表面。将GOX固定在MWCNTs/Pt电极上后，催化氧化电流从约100 V提高到约100 V。-0.45 V（vs. Ag/AgCl/sat'd KCl），接近于生理pH值下GOX辅基黄素腺嘌呤二核苷酸（FAD）的氧化还原电位。此外，果糖脱氢酶（FDH）固定的多壁碳纳米管/铂电极表现出良好的催化氧化电流的基础上FDH观察到约。-0.15V（vs. Ag/AgCl/饱和KCl），这接近于作为FDH辅基的血红素c的氧化还原电位。从催化电流对底物的曲线图的分析，果糖浓度的校准范围高达约100 μ g/ml。40μmol dm^<-3>，并且表观米氏常数被评估为11（±）mmol dm^<-3>关于酶和MWCNT之间的相互作用和直接非均相电子转移反应的进一步研究正在进行中，以开发传感器应用和葡萄糖-氧生物燃料电池。