Development of recognition device for biomolecules using polymer gel-QCM conjugate

点击翻译按钮获取中文摘要

First, method to synthesized micro size hydrogels on the surface of quartz crystal microbalance, QCM, was developed using N-isopropylacrylamide and its copolymer. The gel physically attached on the surface of QCM worked well as a visco-elastic body under the vibration of the quartz. The gold electrode was preferable for the physical attachment of microgel on the QCM. The method to introduce bovine serum albumin was developed. The protein was introduced into the polymer network of gel chemically through covalent bonding. The phase behavior and QCM response of the microgel were investigated as a function of solution pH, ionic strength and solution temperature. The QCM with 5MHz basic resonation responded with high sensitivity to the environmental change quickly. The change in resonation frequency and viscosity term of the QCM appeared due to the visco-elasitic property change of the gel in the environment. The gel contact with very small amount of organic molecules responded for a wide variety of concentration of the organic molecules. When a anti-body of introduced proteins interacted with the protein, the visco-elasticity of the gel responded sensitively with change in resonation of the QCM. The results obtained in the present study indicated that the micro gel coupled with the QCM was potential device for molecular sensing with high sensitivity.