Evolutionary analysis on regulatory mechanism of the Dlx3-7 gene cluster regarding evolution of morphological novelty.

Dlx3-7基因簇形态新颖性进化调控机制的进化分析。

• 批准号: 18570007
• 负责人: SUMIYAMA Kenta
• 金额: $ 2.5万
• 依托单位: National Institute of Genetics
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18570007/
• 关键词: Development; Differentiation; Regulation of gene expression; Evolution; Gene; Genome; Transgenic mice; Evo-devo; cis-element

The mammalian D1×3 and D1×7 genes are organized as a bigene cluster, and their respective expression patterns are controlled temporally and spatially by cis-elements that have been identified in the intergenic region of the cluster. Previous work showed that one of the conserved elements (137-2) was indeed a visceral arch enhancer that was able to recapitulate the nested expression pattern. We have extended these analyses to include twelve additional mammalian taxa (including a marsupial and a monotreme) in order to study evolution of the non-coding functional elements in mammals. Large-scale sequence comparisons and subsequent conservative motif extraction by the evolutionary rigidity assay (ERA) showed that each cis-element motif exhibited different degrees of evolutionary conservation among mammalian species. 137-1 harbored IL very old conservative motif that was shared by amphioxus distal-less gene. On the other hand, 137-2 exhibited less conservation between monotreme and marsupial/eutherian ancestor despite being highly conserved among marsupial/eutherian species. Since 137-2 has been shown to be important for the craniofacial expression, this intermittent evolution of 137-2 element may possibly be related to craniofacial innovation of mammals.

哺乳动物的D1×3和D1×7基因被组织成一个双基因簇，它们各自的表达模式在时间和空间上都受到已在该簇基因间区域鉴定的顺式元件的控制。先前的工作表明，保守元件之一（137-2）确实是一个内脏弓增强子，能够概括嵌套表达模式。我们已经扩展了这些分析，包括12个额外的哺乳动物类群（包括有袋动物和单孔目动物），以研究哺乳动物的非编码功能元件的进化。大规模的序列比较和随后的保守基序提取的进化刚性分析（ERA）表明，每个顺式元件基序表现出不同程度的进化保守哺乳动物物种。137-1具有与文昌鱼无末端基因相同的IL非常古老的保守基序。另一方面，尽管137-2在有袋动物/真兽目物种中高度保守，但在单孔目和有袋动物/真兽目祖先之间表现出较少的保守性。由于137-2在颅面表情中起重要作用，137-2的这种间歇性进化可能与哺乳动物颅面的创新有关。

项目成果

Possible involvement of SINEs in mammalian-specific brain formation

• DOI: 10.1073/pnas.0709398105
• 发表时间: 2008-03-18
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Sasaki, Takeshi;Nishihara, Hidenori;Okada, Norihiro
• 通讯作者: Okada, Norihiro

Identification and evolutionary origin of a limb enhancer in the Dlx3-7 bigene cluster

Dlx3-7 双基因簇中肢体增强子的鉴定和进化起源

• 发表时间: 2007
• 作者: Hiroshi Masuya;Hideki Sezutsu;Yoshiyuki Sakurba;Tomoko Sagai;Masaki Hosoya;Hideki Kaneda;Ikuo Miura;Kimio Kobayashi;Kenta Sumiyama;Aya Shimizu;Junko Nagano;Haruka Yokoyama;Satoko Kaneko;Noriko Sakurai;Yuka Okagaki;Tetsui Noda;Shigeharu Wak;隅山 健太
• 通讯作者: 隅山 健太

ほ乳類Dlx3-7遺伝子クラスター発現調節領域に起きた過去の急速な進化

哺乳动物 Dlx3-7 基因簇表达调控区的快速进化

• 发表时间: 2006
• 作者: Sumiyama K.;Ruddle F.;Sumiyama K;隅山 健太
• 通讯作者: 隅山 健太

A series of ENU-induced single-base substitutions in a long-range cis-element altering Sonic hedgehog expression in the developing mouse limb bud

• DOI: 10.1016/j.ygeno.2006.09.005
• 发表时间: 2007-02-01
• 期刊: GENOMICS
• 影响因子: 4.4
• 作者: Masuya, Hiroshi;Sezutsu, Hideki;Shiroishi, Toshihiko
• 通讯作者: Shiroishi, Toshihiko

Identification and evolutionary origin of a limb enhancer in the D1x3-7 bigene cluster.

D1x3-7 双基因簇中肢体增强子的鉴定和进化起源。

• 发表时间: 2007
• 作者: Sumiyama K.;Ruddle F.
• 通讯作者: Ruddle F.