Mechanism and role of phagocytosis of dying cells and microbes

死亡细胞和微生物吞噬作用的机制和作用

• 批准号: 18570123
• 负责人: SHIRATSUCHI Akiko
• 金额: $ 2.53万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18570123/
• 关键词: phagocytosis; apoptosis; signal transduction; microbes; spermatogenesis; innate immunity; Toll-like receptor; influenza virus; Toll様受容体; 貧食; スカベンジャー受容体

1) Mechanism and role of SR-BI-mediated apoptotic spermatogenic cells by Sertoli cells in the testis. After phagocytosis of apoptotic spermatogenic cells, testicular somatic Sertoli cells produced unknown factor(s) by which spermatogenic cells could differentiate into spermatozoa. To identify the factor, we selected one candidate protein by proteome analysis of Sertoli cells and identified its primary structure. We found that the expression of the factor was stimulated in transcriptional level during phagocytosis. We have been generating knockout mice of the factor, and we are going to examine the role of the factor on spermatogenesis in mice.2) Role of TLR-JNK pathway on bacterial survival in macrophages Staphylococcus aureus induced JNK phosphorylation in mouse peritoneal macrophages depending on toll-like receptor TLR2. This activation of INK pathway caused inhibition of superoxide production in the macrophages, and this allowed engulfed S. aureus to survive in the macrophages.3) Role of phagocytosis of influenza virus-infected cells by macrophages Influenza virus-infected cells produced macrophage-activating factor that enhance macrophage phagocytosis of virus-infected cells depending on both virus infection and apoptosis. This factor would be heat-labile material since it lost the activity after heat-treatment.

1)SR-BI介导睾丸支持细胞凋亡的机制及作用睾丸支持细胞吞噬凋亡的生精细胞后，产生未知因子，使生精细胞分化为精子。为了鉴定该因子，我们通过支持细胞的蛋白质组分析选择了一个候选蛋白，并鉴定了其一级结构。我们发现，在吞噬过程中，该因子的表达在转录水平上受到刺激。我们已经产生了该因子的敲除小鼠，我们将检查该因子在小鼠精子发生中的作用。2）TLR-JNK通路对巨噬细胞中细菌存活的作用金黄色葡萄球菌依赖于Toll样受体TLR 2诱导小鼠腹腔巨噬细胞中JNK磷酸化。这种INK途径的激活导致巨噬细胞中超氧化物产生的抑制，这使得吞噬S。3）巨噬细胞对流感病毒感染细胞的吞噬作用流感病毒感染的细胞产生巨噬细胞活化因子，该因子依赖于病毒感染和细胞凋亡增强病毒感染细胞的巨噬细胞吞噬作用。该因子将是热不稳定材料，因为其在热处理后失去活性。

项目成果

Isolation of an endoplasimic reticulum protein as a candidate ligand for Draper, a Drosophila phagocytosis receptor

分离内质网蛋白作为果蝇吞噬受体 Draper 的候选配体

• 发表时间: 2007
• 作者: Kuraishi;T;Yamamoto;N.;Shiratsuchi;A.;Yoshinobu Nakanishi;他4名
• 通讯作者: 他4名

Pattern recognition in phagocytic clearance of altered self. In: Target Pattern Recognition in Innate Immunity, chapter 11

吞噬清除改变自我的模式识别。

• 发表时间: 2006
• 作者: Nakanishi;Y.;Henson;P. M.;and Shiratsuchi;A
• 通讯作者: A

Mechanisms and roles of phagocytosis in invertebrates.

无脊椎动物吞噬作用的机制和作用。

• 发表时间: 2008
• 期刊: Invertebrate Survival Journal (in press)
• 作者: Nakanishi;Y.;and Shiratsuchi;A
• 通讯作者: A

Perturbation of spermatogenesis by androgen antagonists directly injected into seminiferous tubules of live mice

直接注射到活小鼠生精小管中的雄激素拮抗剂对精子发生的干扰

• 发表时间: 2007
• 期刊: Reproducrion 133
• 作者: Nagaosa;K.;Kishimoto;A.;Kizu;R.;Nakagawa;A. Shiratsuchi;A.;and Nakanishi;Y.
• 通讯作者: Y.

Nakanishi Regulation o : Dectin-l-mediated phagocytosis by cannabinoid receptor CB2

Nakanishi 调节 o：Dectin-l 介导的大麻素受体 CB2 的吞噬作用

• 发表时间: 2007
• 作者: A. Shiratsuchi;I. Watanabe;Y
• 通讯作者: Y