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Genetic studies on the interaction between base excision repair and recombinational repair using human gene knockout cells

Genetic studies on the interaction between base excision repair and recombinational repair using human gene knockout cells
使用人类基因敲除细胞进行碱基切除修复与重组修复之间相互作用的遗传学研究
批准号:
18570163
负责人:
KOYAMA Hideki
金额:
$2.53万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2006
资助国家:
日本
项目状态:
已结题
起止时间:
2006 至 2007

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中文摘要
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英文摘要
In this study, we have performed genetic analysis on the interaction between base excision repair and recombinational repair in human cells. Specifically, we disrupted, by means of gene targeting using Nalm-6 cells, the PolB, Rad54, and Lig4 genes involved in base excision repair homologous recombination, or nonhomologous end-joining, respectively. Additionally, we tried to make double-knockout mutant cell lines for those genes. We investigated the growth rate, cell cycle distributions, and recombination capacity of these mutants. Furthermore, using these mutants, we have successfully analyzed cellular sensitivity to a variety of genotoxic agents such as X-rays, UV light, alkylating agents, hydrogen peroxide, topoisomerase inhibitors, and replication stress. These studies led us to conclude that homologous recombination is important for repairing DNA double-strand breaks that arose from single-strand breaks. Most importantly, this work has highlighted the genetic interaction between DNApolymerase beta and DNAligase W in human cells.
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Enhanced gene targerting efficiency in siRNA that silences the expression of the Bloom syndrome gene in human cells.
增强 siRNA 的基因靶向效率,沉默人类细胞中布卢姆综合征基因的表达。
DOI: --
发表时间: 2006
期刊: Genes to Cells 11
影响因子: --
作者: [So, S.]
通讯作者: S.
The human pre-B cell line Nalm-6 in highly proficient in gene targeting by homologous recombination.
人类前 B 细胞系 Nalm-6 非常擅长通过同源重组进行基因靶向。
DOI: --
发表时间: 2006
期刊: DNA Cell Biol. 25
影响因子: --
作者: [Adachi, N.]
通讯作者: N.
NK314 is a topoisomerase IIalpha specific inhibitor forming stable DNA cleavage complex
NK314 是一种拓扑异构酶 IIα 特异性抑制剂,可形成稳定的 DNA 切割复合物
DOI: --
发表时间: 2007
期刊:
影响因子: --
作者: [Toyoda, E., et. al.]
通讯作者: et. al.
DOI: 10.2144/000112233
发表时间: 2006-09-01
期刊: BIOTECHNIQUES
影响因子: 2.7
作者: [Iiizumi, Susumu, Nomura, Yuji, Koyama, Hideki]
通讯作者: Koyama, Hideki
30
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