Differential gene expression in tthat occurs during the conversion from symmetrically ddividing neuroepithelial stem cells into asymmetricallly dividing neural progenitos

从对称分裂的神经上皮干细胞转变为不对称分裂的神经祖细胞期间发生的差异基因表达

• 批准号: 18570207
• 负责人: NAKAO Keiko
• 金额: $ 2.63万
• 依托单位: Saitama Medical University (2007)Keio University (2006)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18570207/
• 关键词: neuroepithelial stem cells; neural progenitors; cell adhesion; cell cycle; asymmetric division; Symmetric division; interphase; conversion; 対称分裂; 非対称分裂; 上皮間充織転換

The neuroepithelium of the outer proliferating center (OPC) of the Drosophila larval optic lobe comprises the primordia for optic neurons. The neural precursors undergo enormous expansion probably before and sometimes even after differentiating into neuroblasts (NBs), which ultimately produce optic neurons. However, no study has yet provided a detailed explanation of how this expansion occurs. Here we show that neuroepithelial cells (NEs) at the margin of the core neuroepithelial region of the OPC convert individually into NBs during G1 phase. As the cell cycle progresses from G1 to the S phase, the transition is completed. During this G1 period, a cell that used to be an NE becomes committed to differentiate, alters its cell phase control, and changes its morphology from a columnar to a drop-like shape by reorganizing the adherens junctions that had linked it to other NE cells, to finally become an NB and commence asymmetric division.

果蝇幼虫视叶外增殖中心(OPC)的神经上皮构成视神经元的原基。神经前体在分化成神经母细胞(NBS)之前甚至有时甚至在分化成神经母细胞(NBS)之后经历巨大的扩张，最终产生视神经元。然而，还没有一项研究对这种扩张是如何发生的提供详细的解释。在这里，我们展示了位于OPC核心神经上皮区边缘的神经上皮细胞(NE)在G1期单独转化为NBS。随着细胞周期从G1期进入S期，这一转变就完成了。在这个G1期，曾经是NE的细胞开始致力于分化，改变其细胞时相控制，并通过重组连接它与其他NE细胞的贴壁连接将其形态从柱状改变为液滴状，最终成为NB并开始不对称分裂。

项目成果

Neuroepithelial stem cells in the Drosophila optic lobe are converted into neural progenitors during the G1 interphase in a cell cycle

果蝇视叶中的神经上皮干细胞在细胞周期的 G1 间期转化为神经祖细胞

• 发表时间: 2007
• 作者: Kishimoto;Y.;Koshida;S.;Furutani-Seiki;M.;Kawakami;A.;Reiss;J.;Kondoh;H.;Kawakami;K.;岸本 康之;Ozawa Y;織原-小野美奈子;Orihara-Ono M
• 通讯作者: Orihara-Ono M

網膜視細胞の分化調節機構-SOCS3は視細胞分化を開始させる

视网膜感光细胞分化的调控机制——SOCS3启动感光细胞分化

• 发表时间: 2007
• 作者: Kishimoto;Y.;Koshida;S.;Furutani-Seiki;M.;Kawakami;A.;Reiss;J.;Kondoh;H.;Kawakami;K.;岸本 康之;Ozawa Y;織原-小野美奈子;Orihara-Ono M;小沢洋子
• 通讯作者: 小沢洋子

神経上皮性幹細胞から神経前駆細胞への転換機構

神经上皮干细胞向神经祖细胞转化的机制

• 发表时间: 2007
• 作者: Kishimoto;Y.;Koshida;S.;Furutani-Seiki;M.;Kawakami;A.;Reiss;J.;Kondoh;H.;Kawakami;K.;岸本 康之;Ozawa Y;織原-小野美奈子
• 通讯作者: 織原-小野美奈子

Roles of STAT3/SOCS3 pathway in regulating the visual function and ubiquitin-proteasome-dependent degradation of rhodopsin during retinal inflammation.

• DOI: 10.1074/jbc.m802238200
• 发表时间: 2008-09-05
• 期刊: The Journal of biological chemistry
• 作者: Ozawa Y;Nakao K;Kurihara T;Shimazaki T;Shimmura S;Ishida S;Yoshimura A;Tsubota K;Okano H
• 通讯作者: Okano H