Characterization of side population cells in human gastric cancer

应用流式细胞术分析人胃癌（GC）原代培养细胞（MK 7313、MK 8515、MK 8213）的SP细胞分离，所有GC原代培养物均显示出不同比例的SP细胞，范围为0.04%至0.20%。我们证明了SP细胞的干细胞样特征作为富集CSC的可能亚群，如ABC转运蛋白基因表达（ABCC1和ABCG2）、体内化学抗性和致瘤性所示。这些SP细胞在体内表现出比母体组织中的总细胞群更高的致瘤性。总之，我们证明了实体瘤组织如人GC含有CSC，在恶性肿瘤中存在异质性和不同的层次结构，这表明未来可能有一种新的治疗工具靶向CSC以克服这种恶性疾病。

Human gastric cancer (GC) primary culture cell (MK7313, MK8515, MK8213) were analyzed using flow cytometry for SP cell isolation, and all GC primary culture showed a distinct fraction of SP cells, ranging from 0.04 to 0.20%. We demonstrated stem cell-like characteristics of SP cells as a possible subpopulation with enriched CSCs, as indicated by ABC transporter gene expression (ABCC1 and ABCG2), chemo-resistance and tumorigenicity in vivo. These SP cells demonstrate higher tumorigenicity in vivo than does the overall cell population in the parent tissue. In conclusion, we demonstrate that solid tumor tissue such as human GC contains CSCs, with the existence of heterogeneity and distinct hierarchy in malignancy, suggesting the future possibility of a novel therapeutic tool targeting CSCs for overcoming this malignant disease.