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Morphological analysis of the intracolonic lymphatic vessels with reference to the mechanism of lymphatic metastasis

结肠内淋巴管形态学分析并参考淋巴转移机制

基本信息

批准号：
18590170
负责人：
MIURA Masahiro
金额：
$ 2.54万
依托单位：
Oita University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

项目摘要

Previously, we investigated the fine structure and distribution of the lymphatic networks in several mammalian tissues using an enzyme-histochemical method with 5'-nucleotidase (5'-Nase) -alkaline phosphatase (ALPase) double staining to distinguish lymphatics and blood capillaries. Our new histochemical technique clearly demonstrated precise distribution of the lymphatic networks.In the present study, the fine distribution and structure of intramucosal lymphatics in the colon of the Japanese monkey (Macacafuscata) and human were examined mainly enzyme-histochemically with light and electron microscopy. Whole mount preparations of each tissue layer pealed from the colonic wall and cryostat sections were stained with 5'-Nase-ALPase double staining and immunostaining (LYVE-.1, D2-40).5'-Nase-positive lymphatics appeared extensive networks in the lamina propria mucosae (LPM), lamina submucosae (MS), muscularis propria (MP) and subserosal layers, and some of them were found to be closely as … More sociated with ALPase-positive blood vessels. Double-layered lymphatic networks were locally observed near the inner and outer surfaces of lamina muscularis mucosae (LMM). Numerous slender lymphatics without valve-like structures which coursed vertically, were seen in the intercryptal region. All the lymphatics were originated from horizontal networks in the deep LPM. In general, they had scarcely any communicating branch in the superficial layer of LPM except in the cecum and recto-sigmoid colon. In the cecum and recto-sigmoid colon, fine dense lymphatic networks were locally observed in the most superficial layer of LPM. On the other hand, lymphatic networks of the deep LPM were characterized by a regular mesh-like network on a basis of the penetrating point on the LMM. These lymphatic networks closely communicated with a irregular horizontal one which consisted of tubulo-saccular lymphatics and directly located under the LMM via the penetrating branch. The latter showed a lymphatic network with obvious valve-like structures and had numerous enlarged blind-endings.The present study has first demonstrated enzyme-histochemically the precise distribution of 5'-Nase-positive and/or LYVE-1 -positive initial lymphatics by using whole mount preparations in the laminar layer of the monkey and human colons. Our findings suggest that rich initial lymphatics in LPM may play an important role in absorption of the tissue fluid and various free cells. Less

在此之前，我们使用5'-核苷酸酶(5'-Nase) -碱性磷酸酶（ALPase）双染色的酶组织化学方法研究了几种哺乳动物组织中淋巴网络的精细结构和分布，以区分淋巴管和毛细血管。我们新的组织化学技术清楚地显示了淋巴网络的精确分布。本研究主要用光镜和电镜对日本猴和人结肠粘膜内淋巴管的精细分布和结构进行了酶组织化学研究。从结肠壁和低温恒温器切片上撕取的每一组织层的整个挂载制备用5'-Nase-ALPase双染色和免疫染色（LYVE- 1, D2-40）进行染色。5′- nase阳性淋巴管在粘膜固有层（LPM）、黏膜下层（MS）、固有肌层（MP）和浆膜下层出现广泛的网络，其中部分与alpase阳性血管密切相关。粘膜肌层（LMM）内外表面局部可见双层淋巴网络。隐窝间可见许多细长的淋巴管，无瓣状结构，垂直排列。所有淋巴管均起源于LPM深部的水平网络。一般来说，除了盲肠和直乙状结肠外，LPM的浅层几乎没有交通分支。在盲肠和直乙状结肠，在LPM最浅层局部可见细而致密的淋巴网络。另一方面，深层LPM的淋巴网络以LMM的穿透点为基础，具有规则的网状网络特征。这些淋巴网络与一个不规则的水平淋巴网络紧密相连，该淋巴网络由小管-囊状淋巴管组成，通过穿透支直接位于LMM下方。后者表现为具有明显瓣状结构的淋巴网络，并有许多增大的盲末梢。本研究首次用酶组织化学方法证明了5'- nase阳性和/或LYVE-1阳性的初始淋巴管在猴和人结肠的层流层中的精确分布。我们的研究结果表明，LPM中丰富的初始淋巴管可能在组织液和各种游离细胞的吸收中起重要作用。少