Morphological analysis of the intracolonic lymphatic vessels with reference to the mechanism of lymphatic metastasis

在此之前，我们研究了几种哺乳动物组织中淋巴管网络的精细结构和分布，使用酶组织化学方法与5'-核苷酸酶（5'-Nase）-碱性磷酸酶（ALP-N）双重染色，以区分毛细血管和毛细血管。本研究应用酶组织化学方法，用光镜和电镜观察了日本猕猴（Macacafuscata）和人结肠粘膜内淋巴管的微细分布和结构。取结肠壁各层组织标本，用5'-Nase-ALcase双染和免疫组化染色（LYVE-1，D2 - 40），发现5'-Nase阳性染色在粘膜固有层（LPM）、粘膜下层（MS）、固有肌层（MP）和浆膜下层呈广泛的网状分布，其中有些与粘膜下层紧密相连，而另一些则与粘膜下层紧密相连。 ...更多信息 与碱性磷酸酶阳性的血管有关粘膜肌层（LMM）内、外表面局部可见双层淋巴管网。在隐窝间区可见许多细长的无瓣状结构的神经纤维，垂直走行。所有的网络都起源于LPM深层的水平网络。除盲肠和直肠乙状结肠外，LPM浅层几乎无交通分支。在盲肠和直肠乙状结肠中，在LPM的最浅表层中局部观察到细小致密的淋巴管网络。另一方面，深LPM的淋巴网络的特点是一个规则的网状网络的基础上的穿透点LMM。这些淋巴管网通过穿分支与位于LMM正下方的不规则水平淋巴管网紧密相连。本研究首次用酶组织化学方法证实了5 ′-Nase阳性和/或LYVE-1阳性起始淋巴细胞在猴和人结肠层流层的精确分布。结果提示，LPM中丰富的初始代谢产物可能在组织液和各种游离细胞的吸收中起重要作用。少

Previously, we investigated the fine structure and distribution of the lymphatic networks in several mammalian tissues using an enzyme-histochemical method with 5'-nucleotidase (5'-Nase) -alkaline phosphatase (ALPase) double staining to distinguish lymphatics and blood capillaries. Our new histochemical technique clearly demonstrated precise distribution of the lymphatic networks.In the present study, the fine distribution and structure of intramucosal lymphatics in the colon of the Japanese monkey (Macacafuscata) and human were examined mainly enzyme-histochemically with light and electron microscopy. Whole mount preparations of each tissue layer pealed from the colonic wall and cryostat sections were stained with 5'-Nase-ALPase double staining and immunostaining (LYVE-.1, D2-40).5'-Nase-positive lymphatics appeared extensive networks in the lamina propria mucosae (LPM), lamina submucosae (MS), muscularis propria (MP) and subserosal layers, and some of them were found to be closely as … More sociated with ALPase-positive blood vessels. Double-layered lymphatic networks were locally observed near the inner and outer surfaces of lamina muscularis mucosae (LMM). Numerous slender lymphatics without valve-like structures which coursed vertically, were seen in the intercryptal region. All the lymphatics were originated from horizontal networks in the deep LPM. In general, they had scarcely any communicating branch in the superficial layer of LPM except in the cecum and recto-sigmoid colon. In the cecum and recto-sigmoid colon, fine dense lymphatic networks were locally observed in the most superficial layer of LPM. On the other hand, lymphatic networks of the deep LPM were characterized by a regular mesh-like network on a basis of the penetrating point on the LMM. These lymphatic networks closely communicated with a irregular horizontal one which consisted of tubulo-saccular lymphatics and directly located under the LMM via the penetrating branch. The latter showed a lymphatic network with obvious valve-like structures and had numerous enlarged blind-endings.The present study has first demonstrated enzyme-histochemically the precise distribution of 5'-Nase-positive and/or LYVE-1 -positive initial lymphatics by using whole mount preparations in the laminar layer of the monkey and human colons. Our findings suggest that rich initial lymphatics in LPM may play an important role in absorption of the tissue fluid and various free cells. Less