MORPHOLOGICAL ANALYSIS OF THE LYMPHATIC DRAINAGE OF THE ASCiTIC FLUID AND THE METASTATIC MECHANISM OF PERITONEAL

腹水淋巴引流形态学分析及腹膜转移机制

• 批准号: 14570018
• 负责人: MIURA Masahiro
• 金额: $ 1.86万
• 依托单位: OITA UNIVERSITY (FACULTY OF MEDICINE)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14570018/
• 关键词: emzyme-histochemistry; 5'-nucleotidase; lymphatic vessels; parietal peritoneum; whole-mount preparation; monkey; lymphatic metastasis; リンパ管新生; 酵素組織化学; Whole-mount preparation; 中皮下リンパ管系; 新生リンパ管; ダグラス窩

Absorptive pathway via the lymphatic vessels system in the parietal peritoneum is clinically important for understanding lymphatic metastasis in the abdominal and pelvic cavities. Moreover, morphological characteristics of lymphatic networks in the prietal peritoneum have not yet been clarified. Previously we demonstrated enzyme-histochemically the structural organization of the lymphatics and blood capillary in several mammalian tissues. The present study is to describe the structure and distribution of the parietal peritoneal lymphatics in the whole-mount preparations or OCT frozen sections using histochemical method 5'-nucleotidase (5'-Nase) -alkaline phosphatase (ALPase) and intraperitoneal injection with ultra-fine carbon particles (CH-40, 21nm). Fifteen Japanese monkeys (Macaca fuscata) were used in this study. The whole-mount preparation with 5'-Nase -ALPase double staining enabled us to differentiate clearly lymphatic capillaries from the blood capillaries. Well-developed 5'-Na … More se-positive lymphatic networks were locally observed in the transitional area from the diaphragm to the peiitoneal regions and in the perirenal fat region. These tubo-saccular lymphatics were characterized by an irregular mesh-like network. The number of peritoneal lymphatics and their networks in the anterior and anterolateral regions rapidly decreased as compared with that in the foregoing regions. In the rectovesical and rectouterina (Douglas pouch) pouches, a tree-like network consisting of the tubulo-saccular lymphatics was observed. Numerous knob-like endings appeared at the apical parts of the initial lymphatics in the anterior peritoneal region. In Douglas pouch, a fine dense lymphatic network was well-developed around the suspensory ligament of ovary. The present study has first demonstrated enzyme-histochemically the precise distribution of 5'-Nase-positive lymphatic vessels in the monkey parietal peritoneum. These findings suggest a possibility that the peiitoneal lymphatic networks, as an additional pathway may clinically play an important role in absorption of ascitic fluid and particulate matter from the peritoneal cavity. Less

通过壁层腹膜淋巴管系统的吸收途径对于了解腹腔和盆腔的淋巴转移具有临床重要意义。此外，腹膜前叶淋巴网络的形态特征尚未阐明。此前，我们通过酶组织化学方法证明了几种哺乳动物组织中淋巴管和毛细血管的结构组织。本研究采用组织化学方法5'-核苷酸酶（5'-Nase）-碱性磷酸酶（ALPase）和腹腔注射超细碳颗粒（CH-40，21nm）来描述全包埋制剂或OCT冰冻切片中壁层腹膜淋巴管的结构和分布。本研究使用了 15 只日本猴（Macaca fuscata）。使用 5'-Nase-ALPase 双染色的整体制备使我们能够清楚地区分毛细淋巴管和毛细血管。在从膈肌到腹膜区域的过渡区域以及肾周脂肪区域局部观察到发育良好的 5'-Na … More se 阳性淋巴网络。这些管囊淋巴管的特征是不规则的网状网络。与上述区域相比，前部和前外侧区域的腹膜淋巴管及其网络数量迅速减少。在直肠膀胱和直肠外囊（道格拉斯袋）中，观察到由管状淋巴管组成的树状网络。腹膜前区初始淋巴管顶端出现许多旋钮状末端。道格拉斯储袋中，卵巢悬韧带周围有发达的细密淋巴网。本研究首次通过酶组织化学方法证明了猴壁层腹膜中 5'-Nase 阳性淋巴管的精确分布。这些发现表明，腹膜淋巴网络作为额外的途径可能在临床上在腹水和颗粒物从腹膜腔的吸收中发挥重要作用。较少的

项目成果

三浦真弘, 紀 瑞成, 下田 浩, 加藤征治: "直腸子宮窩領域の腹膜リンパ管の微細分布とリンパ流"Japanese Research Society of Clinical Anatomy. 2. 52-53 (2002)

Masahiro Miura、Mizunari Ki、Hiroshi Shimoda、Seiji Kato：“直肠子宫窝区域腹膜淋巴管的精细分布和淋巴流动”日本临床解剖学研究会 2. 52-53 (2002)。

Miura M., Ji R.C, Shimoda H., Kato S.: "Demonstration of lymphatic networks pelvic peritoneum during pregnancy : An enzyme-histochemical observation"Lymphology. 35. 71-75 (2002)

Miura M.、Ji R.C、Shimoda H.、Kato S.：“妊娠期间盆腔腹膜淋巴网络的演示：酶组织化学观察”淋巴学。

三浦真弘, 紀 瑞成, 加藤征治: "Morphological features of the initial portion of the intramural lymphatic vesscls in the large intestines : An enzyme-histochemical and electron microscopic observation"Japanese Research Society of Clinical Anatomy. 3. 48-49 (2003)

Masahiro Miura、Mizunari Ki、Seiji Kato：“大肠壁内淋巴管初始部分的形态特征：酶组织化学和电子显微镜观察”日本临床解剖学研究会 3. 48-49 (2003)。

三浦真弘, 紀瑞成, 加藤征治: "Morphological features of the initial portion of the intramural lymphatic Vessels is the large intestine : An enzyme-histochemical and electron microscopic"Japanese Research Society of Clinical Anatomy. 3. 48-49 (2003)

Masahiro Miura、Mizunari Ki、Seiji Kato：“壁内淋巴管的初始部分是大肠的形态特征：酶组织化学和电子显微镜”日本临床解剖学研究会（2003）。

M.MIURA, RC.JI, H.SHIMODA, S.KATO: "Demonstration of lymphatic networks In monkey pelvic peritoneum during pregnancy : An enzyme-histochemical observation."Lymphology. 35(suppl.). 71-75 (2002)

M.MIURA、RC.JI、H.SHIMODA、S.KATO：“妊娠期间猴盆腔腹膜淋巴网络的演示：酶组织化学观察。”淋巴学。