A Trial to Establish New Methods for the Evaluation of Bio-affinity of Metal Surfaces by using Cell Culture System

点击翻译按钮获取中文摘要

The present study aimed to analyze, by using in vitro cell cultures of human osteoblasts, the dynamic cellular behavior on the clinical materials including metal specimens, and to establish new methods for the evaluation of bio-affinity of such materials. The present study has revealed following data. 1) By using a human cell line of osteoblasts (Saos-2), their behavior on the titanium surface was analyzed in the view point of cell spreading and cell motility with a special reference on the surface smoothness (Li C, Gao S, Kobayashi N, et. al., 2006). Furthermore, intracellular signals regulating cell motility were assayed by the same system to show that actin cytoskeleton has a primary role in the cell motility of Saos-2 cells and that the direction of cell motility is thought to be regulated by IP3-kinase signals. 2) By using a primary culture system of human osteoblasts, a new method has been established to evaluate cell motility on clinical materials. When cells were seeded, a glass cover slip was placed in the center of a plastic culture dish and the cover slip was pressed by a weight. After cells were attached on the dish, the cover slip was removed to obtain a cell-free rectangle region, into which cells started to migrate allowing the measurement of cell motility along the time course. After this simple method, cell motility of the primary cultured cells is shown to be analyzed with a significant reproductivity.