Trial to identify membranous protein(s) regulating the planar cell polarity

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Planar cell polarity is known to be established mainly by the signals originated from the Frizzled-1 receptor localized on the plasma membrane, although available information on the ligand molecule for Frizzled-1 is still limited. The present study has aimed to reveal the unknown ligand molecule(s) using Drosophila cell culture system coupled with chimeric molecule (Frizzled-1-Frizzled-2 chimeras) technique. We have established culture cell-based assay system of the target molecules, together with the constructs enabling to express various membranous receptor molecules reported in Drosophila. We are trying to survey receptor molecules to reveal the Frizzled-1-ligand in question.