The Research on the ion channels which are involved in the cytoprotective medianisms of endothelial cells in the atherosclerotic model animal

我们建立了兔内皮细胞的原代培养方法。我们研究了离子通道在细胞保护作用中的作用。我们将两性霉素B应用于通过增加膜通透性而导致细胞损伤的内皮细胞，并测试了不同的离子条件和离子通道阻滞剂。我们发现氯离子通道阻断剂NPPB和DIDS可以抑制两性霉素B引起的内皮细胞损伤。我们还发现其他有机阴离子取代氯离子可以抑制两性霉素B引起的细胞损伤。这些结果提示，在动脉粥样硬化形成过程中，氯离子通过氯离子通道的内流可能导致内皮细胞的损伤。为了回答这个问题，我们对动脉粥样硬化模型兔(KHC兔)应用高NaC_1饮食6个月，以检查动脉粥样硬化的形成。令人惊讶的是我们发现。高NaCl日粮可抑制动脉粥样硬化的形成。我们正在研究这种效应的机制。

We established the primary culture of the endothelial cells from rabbits. We examined the role of ion channels in the cytoprotective effects. We applied amphotericin B on the endothelial cells which caused cell damage by the enhancement of the membrane permeability and tested different ionic conditions and ion channel blockers. We found that Cl- channel blockers, NPPB and DIDS, inhibited endothelial cell damage caused by amphotericin B. We also found that Cl- replacement by other organic anions suppressed cell-damage by amphotericin B. Cellular damage caused by hydrogen peroxide was also inhibited by Cl- channel blockers or Cl- free conditions. These results suggest that Cl- influx via Cl- channels might cause the endothelial cell damage during the formation of atherosclerosis. To access this question, we applied high NaC1 diet to the atherosclerosis model rabbit (KHC rabbit) for 6 months to check the formation of atherosclerosis. Surprisingly we found. that the formation of atherosclerosis was suppressed by the high NaC1 diet. We are investigating the mechanisms of this effect.