Molecular analysis of tissue-specific imprinting

大多数印记基因在其转录的所有组织中都表现出亲本来源特异性基因表达，然而有些基因以组织特异性方式印记。显示大脑特异性印记或大脑特异性印记缺失的基因为研究组织分化过程中的印记过程提供了独特的机会。在这个项目中，我使用原代脑细胞培养系统分析了脑细胞谱系特异性印记，并讨论了神经元中与印记相关的表观遗传修饰。我关注的是印记基因 Grb10 和 Snrpn-Ube3a，它们。显示神经元特异性印记并分析 DNA 甲基化、组蛋白修饰和反义 RNA。

Most imprinted genes display parental-origin-specific gene expression in all tissue where they are transcribed, however some genes are imprinted in a tissue-specific manner. Genes that show brain-specific imprinting or brain-specific lack of imprinting present a unique opportunity to study the process of imprinting during tissue differentiation. In this project, I analyzed brain cell lineage-specific imprinting using a primary brain cell culture system and discussed imprinting-associated epigenetic modifications in neurons. I focused on the imprinted genes, Grb10, and Snrpn-Ube3a, which. show neuron-specific imprinting and analyzed DNA methylation, histone modifications and antisense RNA.