Analysis of Bone Marrow Derived Cells Homing to Gastric Cancer and metastasis

骨髓来源细胞归巢至胃癌和转移的分析

• 批准号: 18590363
• 负责人: NOMURA Sachiyo
• 金额: $ 2.53万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18590363/
• 关键词: Gastric Cancer; stem cell; carcinogenesis; bone marrow; clonality; bone marrow derived cell; esophageal cancer; metaplasia; 骨髄幹細胞; 胃癌転移; 胃発がん; ヘリコバクター; 骨髄移植; 胃粘膜細胞分化; 胃発癌

We analyzed whether gastric cancer is originated from bone marrow derived cells, using mouse chemical carcinogenesis model. Seventeen BALB/c male mice were irradiated 9 Gy and transplanted bone marrow cells extracted from GFP mice. They started drinking water including MNU 240 ppm after recovery from the damage of irradiation. They drank MNU for 5weeks every other week, durated for 9 weeks. 4 mice among 17 were spared for control, and 13 mice were applied MNU. After 40 weeks later from the beginning of taking MNU, they were sacrificed and the stomachs were excised. When the time of sacricficing, only 6 mice out of 13 were alive. Among them, 4 mice beared squamous cell carcinoma in the forestomach. Almost all the cancer cells were GFP negative and they were speculated to be derived from original stomach cells. However, only a small part of cancer cells were GFP positive and they were speculated from bone marrow derived cells. Lymph node metastasis were observed, but, they were GFP negative and there was no homing of bone marrow derived cells.From the results of this experiment, not all the cancer is derived from bone marrow derived cells. However, there is still possibility that small part of cancer is originated from bone marrow derived cells. We need to research more to confirm the results. We could also show the possibility that cancer is not monoclonal.

本研究采用小鼠化学致癌模型，分析胃癌是否起源于骨髓源性细胞。17只BALB/c雄性小鼠接受9戈伊照射，并移植从GFP小鼠中提取的骨髓细胞。他们从辐射损伤中恢复后开始饮用含MNU 240 ppm的水。他们每隔一周喝一次MNU，连续5周，持续9周。17只小鼠中4只作为对照，13只应用MNU。在开始服用MNU 40周后，将它们处死并切除胃。处死时，13只小鼠中仅有6只存活。其中4只小鼠前胃发生鳞状细胞癌。几乎所有的癌细胞都是GFP阴性的，并且推测它们来源于原始胃细胞。然而，只有一小部分癌细胞是GFP阳性的，并且推测它们来自骨髓来源的细胞。观察到淋巴结转移，但是它们是GFP阴性的，并且没有骨髓来源的细胞归巢。从该实验的结果来看，并非所有的癌症都来源于骨髓来源的细胞。然而，仍有一小部分癌症可能起源于骨髓来源的细胞。我们需要进一步研究以证实结果。我们还可以证明癌症不是单克隆的。

项目成果

Expression profiling of transdifferentiation of chief cells into Gastric metaplasia.

主细胞转分化为胃化生的表达谱。

• 发表时间: 2006
• 作者: Masako Ogawa;Sachiyo Nomura;James R. Goldenring;Michio Kaminishi
• 通讯作者: Michio Kaminishi

Effect of Helicobacter Pylori Infection in Experimental Gastric Carcinogenesis of Mongolian Gerbils

幽门螺杆菌感染对沙鼠实验性胃癌发生的影响

• 发表时间: 2006
• 作者: Koji Nozaki;xueyuan Cao;Harunari Tanaka;Yuzuru Ikehara;Tetsuya Tsukamoto;Hayao Nakanishi;Nobuyuki Shimizu;Sachiyo Nomura;Hirokazu Yamaguchi;Yoshio Yamaoka;Michio kaminishi;Masae Tatematsu
• 通讯作者: Masae Tatematsu

Emergence of Spasmolytic Polypeptide-Expressing Metaplasia (SPEM) in Mongolian Gerbils infected with Helicobacterpylori

感染幽门螺杆菌的蒙古沙鼠出现解痉多肽表达化生（SPEM）

• 发表时间: 2007
• 期刊: Lab Invest 87(12)
• 作者: Nao Yoshizawa;Yoshiharu Takenaka;Hirokazu Yamaguchi;Tsukamoto Tetsuya;Tatematsu Masae;Sachiyo Nomura;James R Goldenring;Michio Kaminishi
• 通讯作者: Michio Kaminishi

胃癌における転写調節遺伝子MondoAのスプライシング多型

胃癌转录调控基因MondoA剪接多态性

• 发表时间: 2007
• 作者: 野村幸世;James R. Goldenring;Donald E. Aver;上西紀夫
• 通讯作者: 上西紀夫

Omeprazole treatment ameliorates oxyntic atrophy induced by DMP-777

• DOI: 10.1007/s10620-006-3151-x
• 发表时间: 2006-03-01
• 期刊: DIGESTIVE DISEASES AND SCIENCES
• 影响因子: 3.1
• 作者: Ogawa, M;Nomura, S;Goldenring, JR
• 通讯作者: Goldenring, JR