Fumctional analysis of AEPP in skeletal muscle

骨骼肌AEPP的功能分析

• 批准号: 18590375
• 负责人: MORIYAMA Masatsugu
• 金额: $ 2.57万
• 依托单位: Oita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18590375/
• 关键词: ARPP; CARP; muscle injury; Skeletal muscle; MARP; Nuclear translocation; Sarcomere; I band; ノックアウトマウス; 脂肪酸; FABP; 糖代謝

Ankyrin-repeat protein with a PEST motif and a proline-rich region(Arpp), also designated as Ankrd2, is a member of the muscle ankyrin repeat proteins (MARPs), which have been proposed to be involved in muscle stress response pathways. Arpp/Ankrd2 is localized mainly in the I-band of striated muscle. However, it has recently been reported that Arpp/Ankrd2 can interact with nuclear proteins, such as premyelocytic leukemia protein (PML), p53 and YB- 1 in vitro. In this study, to determine whether nuclear accumulation of Arpp/Ankrd2 actually occurs, we performed an immunohistochemical investigation of gastrocnemius muscles that had been injured by injection of cardiotoxin or contact with dry ice. We found that Arpp/Ankrd2 accumulated in the nuclei of myofibers located adjacent to severely damaged myofibers after muscle injury. Double-labeled immunohistochemistry revealed that Arpp/Ankrd2 accumulated in the nuclei of sarcomere-damaged myofibers. Furthermore, we found that Arpp/Ankrd2 tended to be localized in euchromatin where genes are transcriptionally activated. Based on these findings, we suggest that Arpp/Ankrd2 may translocate from the I-band to the nucleus in response to muscle damage and may participate in the regulation of gene expression.

锚蛋白重复序列蛋白（Ankyrin-repeat protein，Ankrd 2）是肌肉锚蛋白重复序列蛋白（muscle ankyrin repeat proteins，MARP）的一个成员，具有PEST基序和富含脯氨酸的区域（proline-rich region，Arpp），被认为参与肌肉应激反应途径。Arpp/Ankrd 2主要定位于横纹肌I带。然而，最近有报道称Arpp/Ankrd 2可以在体外与核蛋白如早幼粒细胞白血病蛋白（PML）、p53和YB- 1相互作用。在这项研究中，为了确定是否真的发生了Arpp/Ankrd 2的核积聚，我们对注射心脏毒素或与干冰接触而受伤的腓肠肌进行了免疫组织化学研究。我们发现，Arpp/Ankrd 2积累在肌纤维的细胞核位于附近的严重受损的肌纤维肌肉损伤后。双标记免疫组化显示，Arpp/Ankrd 2积累在肌节损伤的肌纤维的细胞核中。此外，我们发现Arpp/Ankrd 2倾向于定位在常染色质中，在那里基因被转录激活。基于这些发现，我们认为Arpp/Ankrd 2可能在肌肉损伤时从I带转位到细胞核，并可能参与基因表达的调节。

项目成果

Title : High-resolution analysis of DNA copy number alterations and the gene expression in renal clear cell carcinoma

标题 : 肾透明细胞癌 DNA 拷贝数改变和基因表达的高分辨率分析

• 发表时间: 2007
• 期刊: Journal of Pathology vol.213
• 作者: T.;Yosbimoto;K.;Matsuura;S.;Karnan;H.;Tagawa;C.;Nakada;M.;Tanigawa;Y.;Tsukamoto;T.;Uchida;K.;Kashima;S.;Akizuki;I.;Takeuchi;F.;Sato;H.;Mimata;M.;Seto;M.;Morivama
• 通讯作者: Morivama

ARPP protein is selectively expressed in renal oncocytoma, but not in real cell carcinomas.

ARPP 蛋白在肾嗜酸细胞瘤中选择性表达，但在真正的细胞癌中不表达。

• 发表时间: 2007
• 期刊: Modern Pathology 20
• 作者: Shimori K;Nagashima Y;Kuroda M;Takeda A;Tsukamoto Y;Kawamura T;Hiramatsu T;Matsuura K;Hijiya N;Yano S;Yokoyama S;Ito H;Moriyama M
• 通讯作者: Moriyama M

Immunohistochemical diagnosis of the cagA-gene genotype of Helicodacter pylori with antj-East Asian CagA-specific antibody.

使用抗东亚 CagA 特异性抗体对幽门螺旋杆菌 cagA 基因基因型进行免疫组织化学诊断。

• 发表时间: 2007
• 期刊: Cancer Science 98
• 作者: Uchida T;Kanada R;Tsukamoto Y;Hijiya N;Matsuura K;Yano S;Yokoyama S;Kishida T;Kodama M;Murakami K;Fujioka T;Moriyama M
• 通讯作者: Moriyama M

Titled Arpp/Ankrd2, a member of the Muscle Ankyrin Repeat Proteins(MARPs), translocates from the Pband to the nucleus after muscle injury

名为 Arpp/Ankrd2 的肌肉锚蛋白重复蛋白 (MARP) 成员，在肌肉损伤后从 Pband 易位至细胞核

• 发表时间: 2008
• 期刊: Histochemistry and Cell Biology vol.129
• 作者: Y.;Tsukamoto;N.;Hijiya;S.;Yano;S.;Yokoyama;C.;Nakada;T.;Uchida;K.;Matsuura;M.;Morivama
• 通讯作者: Morivama

Upregulated expression of cardiac ankyrin-repeated protein in renal podocytes is associated with proteinuria severity in lupus nephritis.

肾足细胞中心脏锚蛋白重复蛋白的表达上调与狼疮性肾炎的蛋白尿严重程度相关。

• 发表时间: 2007
• 期刊: Human Pathology 38
• 作者: Matsuura K;Uesugi N;Hijiya N;Uchida T and Moriyama M
• 通讯作者: Uchida T and Moriyama M