Genome-wide microRNA expression profiling in gastric cancer

胃癌全基因组 microRNA 表达谱

• 批准号: 21590398
• 负责人: MORIYAMA Masatsugu
• 金额: $ 3万
• 依托单位: Oita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21590398/
• 关键词: マイクロアレイ; 網羅的解析; 胃癌; マイクロRNA; microRNA; アポトーシス; アレイCGH; ゲノムコピー数異常; real-time PCR

We investigated expression profiles of microRNA(miRNA) in gastric carcinomas by use of a miRNA microarray platform covering a total of 470 human miRNAs. By comparing miRNA expression profiles between intestinal-type and diffuse-type gastric carcinomas(ANOVA P<0. 05), we could not identify any miRNAs differentially expressed between them, suggesting that, in gastric carcinomas, the miRNA expression profile might not be correlated with histopathologic subtypes. We identified 39 differentially expressed miRNAs in gastric carcinoma, of which 6 were significantly downregulated and the other 33 were upregulated. Among the 6 down-regulated miRNA, we selected 3, miR-375, miR-29c and miR-148a, as gastric cancer-related miRNA.

我们利用覆盖470个人类miRNA的miRNA微阵列平台研究了miRNA在胃癌中的表达谱。通过比较肠型和弥漫型胃癌miRNA表达谱(ANOVA P&lt；0。05)，我们没有发现它们之间有任何差异表达的miRNA，这表明，在胃癌中，miRNA的表达谱可能与组织病理亚型无关。我们鉴定了39个在胃癌中差异表达的miRNAs，其中6个表达显著下调，另外33个表达上调。在6个下调的miRNA中，我们选择了3个miR-375、miR-29c和miR-148a作为与胃癌相关的miRNA。

项目成果

Genome-wide microRNA expression profiling in renal cell carcinoma: significant down-regulation of miR-141 and miR-200c

• DOI: 10.1002/path.2437
• 发表时间: 2008-12-01
• 期刊: JOURNAL OF PATHOLOGY
• 影响因子: 7.3
• 作者: Nakada, C.;Matsuura, K.;Moriyama, M.
• 通讯作者: Moriyama, M.

MicroRNA-375 Is Downregulated in Gastric Carcinomas and Regulates Cell Survival by Targeting PDK1 and 14-3-3ζ

• DOI: 10.1158/0008-5472.can-09-2777
• 发表时间: 2010-03-15
• 期刊: CANCER RESEARCH
• 影响因子: 11.2
• 作者: Tsukamoto, Yoshiyuki;Nakada, Chisato;Moriyama, Masatsugu
• 通讯作者: Moriyama, Masatsugu

MicroRNA-375 is downregulated in gastric carcinomas and regulates cell survival by targeting PDK1 and 14-3-3zeta.

MicroRNA-375 在胃癌中下调，并通过靶向 PDK1 和 14-3-3zeta 来调节细胞存活。

• 发表时间: 2010
• 期刊: Cancer Res. 70
• 作者: Tsukamoto;Y
• 通讯作者: Y

胃癌におけるマイクロRNA発現

胃癌中微小RNA的表达

• 发表时间: 2011
• 作者: 成松隆弘;ほか
• 通讯作者: ほか

Overexpression of miR-210, a downstream target of HIF1α, causes centrosome amplification in renal carcinoma cells

• DOI: 10.1002/path.2860
• 发表时间: 2011-06-01
• 期刊: JOURNAL OF PATHOLOGY
• 影响因子: 7.3
• 作者: Nakada, Chisato;Tsukamoto, Yoshiyuki;Moriyama, Masatsugu
• 通讯作者: Moriyama, Masatsugu