Analysis of biological properties of hepatoma-derived growth factor as a biological marker of lung injury

采用Olympus微采样探针BC-401C采集了无弥漫性肺部疾病（间质性肺炎、结节病、嗜酸性肺炎和血痰患者）105例气道上皮衬里液（ELF）。完成了57例（间质性肺炎27例、结节病4例、嗜酸性肺炎4例、血痰4例、其他疾病18例）标本的分析，总ELF量为30.2±15.3mg。每个病例用三个探针取1ml生理盐水对ELF进行洗脱。ELF中蛋白总量为666.6±700.3 μ g/mL。我们报道了肝癌源性生长因子（HDGF）与肺结节病等炎性疾病的发病机制有关。所有样品均采用ELISA系统检测HDGF， HDGF浓度范围为6.7 ~ 73.5 ng/ml。采用多路悬浮阵列系统27Plex （BioPlex）对两例间质性肺纤维化病例的细胞因子进行检测。检测到IL-1b、-5、-6等16种细胞因子。因此，证实了微采样方法的安全性和有效性，因为没有观察到任何临床显著的不良事件，并且通过该方法为每个病例获得了足够的ELF用于分析。

Airway-epithelial lining fluid (ELF) was obtained by using Olympus micro sampling probe BC-401C from 105 cases of various lung diseases (interstitial pneumonia, sarcoidosis, eosinophilic pneumonia and hemosputum patients without diffuse lung diseases). The analysis of samples of fifty-seven (27 cases of interstital pneumonia, 4 cases of sarcoidosis, 4 cases pf eosinophilic pneumonia, 4 cases of hemosputum and 18 case of other disases) has been completed, and total ELF amount was 30.2±15.3mg. The ELF was eluated by 1 ml of saline from three probes for each case. Total amount of protein in the ELF was 666.6±700.3microg/mL. We have reported that hepatoma-derived growth factor (HDGF) is relevant to the pathogenesis of inflammatory lung diseases such as sarcoidosis. HDGF was detected by ELISA system in all the samples tested, and the range of concentration of HDGF was from 6.7 to 73.5 ng/ml. Cytokine in samples from two interstital pulmonary fibrosis cases was assessed by using Multiplex Suspension Array System 27Plex (BioPlex). Sixteen cytokines such as IL-1b, -5, -6 were detected. Thus, it is confirmed safety and usefulness of the microsampling method, because any clinically significant adverse events were not observed, and sufficient amount of ELF for analysis was obtained by the method for each case.