Analysis of biological properties of hepatoma-derived growth factor as a biological marker of lung injury

肝癌源性生长因子作为肺损伤生物学标志物的生物学特性分析

• 批准号: 18590874
• 负责人: HAYASHI Seiji
• 金额: $ 2.39万
• 依托单位: Clinical Research Center, National Hospital Organization, Kinki-Chuo Chest Medical Center
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18590874/
• 关键词: microsampling; cytokine; hepatoma-derived growth factor; airway-epithelial lining fluid; diffuse lung disease; interstitial pneumonia; multiplex suspension array system; サルコイドーシス; 肝癌由来増殖因子; 好酸球性肺炎; 肺がん; 上皮再生; 上皮傷害

Airway-epithelial lining fluid (ELF) was obtained by using Olympus micro sampling probe BC-401C from 105 cases of various lung diseases (interstitial pneumonia, sarcoidosis, eosinophilic pneumonia and hemosputum patients without diffuse lung diseases). The analysis of samples of fifty-seven (27 cases of interstital pneumonia, 4 cases of sarcoidosis, 4 cases pf eosinophilic pneumonia, 4 cases of hemosputum and 18 case of other disases) has been completed, and total ELF amount was 30.2±15.3mg. The ELF was eluated by 1 ml of saline from three probes for each case. Total amount of protein in the ELF was 666.6±700.3microg/mL. We have reported that hepatoma-derived growth factor (HDGF) is relevant to the pathogenesis of inflammatory lung diseases such as sarcoidosis. HDGF was detected by ELISA system in all the samples tested, and the range of concentration of HDGF was from 6.7 to 73.5 ng/ml. Cytokine in samples from two interstital pulmonary fibrosis cases was assessed by using Multiplex Suspension Array System 27Plex (BioPlex). Sixteen cytokines such as IL-1b, -5, -6 were detected. Thus, it is confirmed safety and usefulness of the microsampling method, because any clinically significant adverse events were not observed, and sufficient amount of ELF for analysis was obtained by the method for each case.

应用Olympus BC-401 C微量取样探针对105例不同肺部疾病（间质性肺炎、结节病、嗜酸性粒细胞性肺炎和无弥漫性肺部疾病的血痰患者）的气道上皮衬里液（ELF）进行了测定。共分析57例（肺炎27例，结节病4例，嗜酸性粒细胞性肺炎4例，血痰4例，其他疾病18例），ELF总量为30.2±15.3mg。对于每种情况，用来自三个探针的1 ml盐水洗脱ELF。ELF中蛋白质总量为666.6± 700.3 μ g/mL。我们已经报道了肝癌衍生生长因子（HDGF）与炎性肺疾病如结节病的发病机制有关。用ELISA方法检测了所有受试样品中的HDGF，其浓度范围为6.7 ~ 73.5ng/ml。通过使用Multiplex Suspension Array System 27 μ m（BioLab）评估来自两个肺纤维化病例的样品中的细胞因子。检测IL-1b、IL-5、IL-6等16种细胞因子。因此，证实了微量采样方法的安全性和有效性，因为未观察到任何临床显著不良事件，并且通过该方法获得了用于分析的足量ELF。

项目成果

マイクロサンプリング法を用いた炎症性肺疾患気道被覆液中の液性因子の定量

使用微量采样方法定量炎症性肺病气道内层液中的体液因子

• 发表时间: 2008
• 作者: R.Zheng;S.Yano;H.Zhang;E.Nakatani;I.Tachibana;I.Kawase;S.Hayashi;S.Sone;林清二
• 通讯作者: 林清二

「特集/肺疾患の炎症のメカニズムとその制御」特発性間質性肺炎

“肺部疾病炎症的特点/机制及其控制”特发性间质性肺炎

• 发表时间: 2006
• 期刊: 呼吸と循環 54
• 作者: 森 雅秀;新井 徹;吉田 光宏;林 清二
• 通讯作者: 林 清二

間質性肺炎-I nterleukin 6 Bench to Bedside

间质性肺炎-白细胞介素 6 从实验室到床边

• 发表时间: 2007
• 作者: 林 清二

CD9 overexpressoin suppresesd the liver metastasis and malignant ascites viainhibition of proliferation and motility of small-cell lung cancer cells in NK-cell depleted SCID mice

CD9 过表达通过抑制 NK 细胞耗尽 SCID 小鼠中小细胞肺癌细胞的增殖和运动来抑制肝转移和恶性腹水

• 期刊: Oncol Res (in press)
• 作者: R.Zheng;S.Yano;H.Zhang;E.Nakatani;I.Tachibana;I.Kawase;S.Hayashi;S.Sone
• 通讯作者: S.Sone

Titration of cytokines in airway epithelial lining fluid of inflammatory lung diseases by microsampling method

微量采样法滴定炎症性肺病气道上皮内层液中的细胞因子

• 发表时间: 2008