The cloning and investigations for antidepressants affected protein with new chemical proteomics approach

由于临床前和临床研究均明确提示5-羟色胺（5-HT）和去甲肾上腺素神经递质系统参与抗抑郁药的作用机制，因此提出了抑郁症病理生理的单胺能假说。我们的研究调查了SSRI、SNRI如何在体内神经元中起作用，以及哪种蛋白质结合抗抑郁药。采用米那西普兰（SNRI）亲和柱，应用大鼠脑细胞质组分。采用二维分析系统可变图像分析仪检测抗抑郁药物结合蛋白，并用质谱法进行鉴定。用共聚焦显微镜检测咪那西普朗键4-氟-7-硝基- 2,1,3 -苯并恶二唑（NBD-F）在PC12细胞中的作用。利用共聚焦显微镜研究了milnacipran键NBD-F与抗抑郁药物结合蛋白的共定位。

Since both preclinical and clinical studies have clearly implicated the serotonin (5-HT) and norepine phrine neurotransmitter systems in the mechanism of action of antidepressants, a monoaminergic hypothesis of the pathophysiology of depression was put forward. Our study investigated how SSRI, SNRI acted in vivo neuron, and which protein bond antidepressants.We used milnacipran (SNRI) hiaffinity colum and applied rat brain cytosol fraction. Antidepressants binding protein was detected the 2D analysis system variable imageanalyzer, and identify by the mass spectrometry.Milnacipran bond 4-Fluoro-7-nitro-2, 1, 3- benzoxadiazole (NBD-F) was detected in vivo PC12 cells by confocal microscope. We investigated that milnacipran bond NBD-F colocalize antidepressants binding protein by confocal microscope.