Transplantation of artificial esophagus using bFGF, keratinocyte, polyglicolic acid felt and polyglactin mesh stent

使用 bFGF、角质形成细胞、聚乙醇酸毛毡和聚乳酸网状支架移植人工食管

• 批准号: 18591403
• 负责人: MOTOYAMA Satoru
• 金额: $ 2.51万
• 依托单位: Akita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18591403/
• 关键词: artificial esophagus; keratinocyte; polyglactin mesh stent; polyglicolic acid felt and; bFGF; 口腔粘膜; ゼラチン

Esophageal replacement using an artificial esophagus is considered difficult. Attempts have been made to replace esophageal defects with a variety of artificial esophagus. In this research, I designed a new type of artificial esophagus, consisted of bFGF-releasing sheet, keratinocyte, polyglicolic acid felt, and polyglactin mesh stent. We successfully developed this new type of artificial esophagus in vitro, and replaced for cervical and thoracic esophagus in dogs. Intravenous hyperalimentation was performed for 4 weeks after the surgery. As results, artificial esophageal tissue successfully replaced the esophageal defect. Microscopically, complete epithelization was observed in the replaced artificial esophagus. However, a stenosis in replaced esophagus occurred frequently. In the thoracic esophageal replacement, all dogs died after surgery within a month due to surgical stress and/or surgical complications. We did not transplant using a new type of artificial esophagus, however, the solution these problems will hold promise for esophageal reconstruction in a clinical setting.

使用人工食管替代食管被认为是困难的。已经尝试用各种人工食管替代食管缺损。本研究设计了一种新型的人工食管，由bFGF释放片、角质形成细胞、聚羟基乙酸毡和聚乳糖网状支架组成。我们成功地在体外研制出了这种新型人工食管，并用于犬颈、胸段食管的替代。术后静脉高营养4周。结果，人工食管组织成功地替代了食管缺损。显微镜下，在替换的人工食管中观察到完全上皮化。然而，替换食管狭窄的发生率很高。在胸段食管置换术中，所有犬均在术后一个月内因手术应激和/或手术并发症死亡。我们没有使用新型人工食管进行移植，然而，这些问题的解决方案将为临床环境中的食管重建提供希望。

项目成果

Surgical outcome of colon interposition via the posterior mediastinal route for thoracic esophageal cancer

后纵隔入路结肠置入术治疗胸段食管癌的手术效果

• 发表时间: 2007
• 期刊: Annals of Thoracic Surgery 83
• 作者: Motoyama;S;et. al.
• 通讯作者: et. al.