A study about the anticancer agent-related gene change in the human lung cancer orthotopic implantation model

人肺癌原位移植模型采用SCID小鼠，形成原发肿瘤，成功制作纵隔淋巴结转移模型。这对于癌症模型来说是罕见的。因此，我们利用ACT-1细胞(甲状腺间变性癌)和YES-3细胞(食道癌)，用SCID小鼠制作了人癌细胞模型。该方法在麻醉下将SCID小鼠固定在面朝上的位置。在食道的情况下，在上腹部增加约1.5 cm的水平切开后关闭胃，手术显露食道，并用30g针在食道肌层注射预先准备的细胞悬液10μml。甲状腺加水平宫颈切开后缝合伤口，显露气管确认甲状腺，用30g针于甲状腺内注射细胞悬液10μml。四周后，该模型在…中形成了微转移超过6周，并在8周内形成大转移。此外，甲状腺癌在注射6周内在甲状腺内发生了肿瘤，并在8周内了解到导致纵隔淋巴结转移。因为能够在小鼠身上复制出人食道癌的淋巴转移，每一个甲状腺癌，因为我做了这些辅助检查，所以现在有了对《癌联》杂志的稿件准备，而且有可能在正确的时间进行一个疗程，使肿瘤转移。另外，我们进行了抗癌剂的剂量，以及抗癌剂在肿瘤相关基因变化的检查，但我们决定在皮下进行它之前，前述的地方移植模型，并制作，5-FU和CDDP给SCID小鼠皮下注射肿瘤，在剂量期间，在抵抗获得的东西之间大约有一个YES-3细胞。我们收集了每个肿瘤组织在有效表达抗癌药剂量之前的耐药获取时间。对于基因去甲基化，我在DNA阵列中进行了搜索。提示PI3K/Akt信号通路与抗性获得有关。我用上述地方移植模型进行了抗癌药物剂量的研究，并将对原位种植模型中抗癌药物连接基因的变化进行研究。较少

The human lung cancer orthotopic implantation model uses an SCID mouse and forms the primary tumor and succeeds in making the mediastinal lymph node metastasis model. It is the rare for the cancer model. Therefore, using ACT-1 cells (anaplastic carcinoma of thyroid) and YES-3 cells (esophageal cancer), we made the human cancer cell models with SCID mouse. The method fixes the SCID mouse in face up position under the anesthesia. We shut stomach after adding about 1.5cm horizontal incision to an epigastric region in the case of the gullet and perform an operation and expose the esophagus, and having injected cell suspension 10 μ ml which we prepared beforehand in the esophageal muscular layer with 30G needle. We shut wound after adding horizontal incision to a cervix in the case of the thyroid gland and expose the trachea and confirm the thyroid gland, and having injected cell suspension 10 μ ml in the thyroid gland with 30G needle. Four weeks later, the model formed micro-metastases in … More six weeks, and formed macro-metastases in eight weeks. In addition, the thyroid carcinoma did neoplasia in the thyroid gland in injection six weeks and understood that caused mediastinum lymph node metastasis in eight weeks. Because it was able to reproduce lymph node metastasis of the cancer of the human esophageal cancer in the mouse, each thyroid carcinoma, there was it of contribution preparations to the magazine of the cancer connection now because I perform these supplementary examinations, and it was possible for a course in correct time in progress to make the metastasis from neoplasia.In addition, we performed the anticancer agent dosage, and it was the examination of the anticancer agent in the tumor-related gene change, but we decided to perform it in a model under the skin before aforementioned place transplant model, and making of, 5-FU and CDDP administered a tumor to the SCID mouse subcutaneous about a YES-3 cell between things of resistant acquisition during the dosage. We gathered each tumor organization at the time of effect expression the anticancer agent dosage ago at resistant acquisition time. For genetic norrowing, I searched it in DNA array. It was suggested that PI3K/Akt signaling pathway was related to resistant acquisition. I perform the anticancer agent dosage with the aforementioned place transplant model mentioned above and am going to perform a study about the anticancer agent connection gene change in the orthotopic implantation model. Less