Development of palminp-sizedbiochemical analysis system based on magnetic beads handling

基于磁珠处理的棕榈大小的生化分析系统的开发

• 批准号: 18310093
• 负责人: SHIKIDA Mitsuhiro
• 金额: $ 10.78万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18310093/
• 关键词: Micro-Nano devices; Micromachine; Biotechnologies

We developed a palmtop-sized total analysis system by using MEMS technologies. The, obtained results are in the followings.(1) We constructed the dynamic model for beads handling, and cleared the relationship between the interface tension and the magnetic driving force. The scale effect for the handling was also explained in the research.(2) We developed the fabrication process of analysis devices by MEMS technologies. The devices were fabricated by the silicone resin, and their all surface was coated by the parylene film to form the hydrophobic nature.(3) We confirmed the biochemical treatments, reaction, extraction, and dilution, on the developed devices by applying magnetic beads handling. We also developed the agitation performance by controlling magnetic field, which was modified by the multilayered flat coils.(4) We confirmed the performance of biochemical reactions by applying the enzymatic reaction, and finally developed the palmtop-sized system by using rotational driving mechanism.

我们利用MEMS技术开发了一个掌上电脑大小的整体分析系统。得到的结果如下。(1)建立了磁珠搬运动力学模型，明确了界面张力与磁驱动力之间的关系。研究还解释了处理的尺度效应。(2)开发了基于MEMS技术的分析器件制造工艺。该器件由有机硅树脂制成，并在其表面涂覆聚对二甲苯膜以形成疏水性。(3)采用磁珠处理技术，对所研制的装置进行生化处理、反应、提取和稀释。我们还开发了通过控制磁场来改善搅拌性能的多层扁平线圈。(4)我们利用酶促反应验证生化反应的性能，最后利用旋转驱动机构开发出掌上大小的系统。

项目成果

Portable Rotary-drive-type Biochemical-Analysis System based on Magnetic bead-droplet Handling

基于磁珠液滴处理的便携式旋转驱动式生化分析系统

• 发表时间: 2007
• 期刊: Tech. Dig. of IEEE Micro Electro Mechanical Systems Conference
• 作者: N.Nagao;M.Shikida;K.Takayanagi;H.Honda;M.Okochi;他2名
• 通讯作者: 他2名

Evaluation of Magnetic Beads Agitation Performance Operated by Multi-layered Flat Coil

多层扁平线圈磁珠搅拌性能评价

• 发表时间: 2007
• 作者: M. Koyama;N. Nagao;R. Imai;M. Shikida;H. Honda;他3名
• 通讯作者: 他3名

Magnetic bead-droplet handling for biochemical reaction in Micro-TAS

Micro-TAS 中生化反应的磁珠液滴处理

• 发表时间: 2006
• 期刊: Tech. Dig. the 23rd Sensor Symposium
• 作者: N.Nagao;M.Shikida;K.Takayanagi;H.Honda;M.Okochi;他2名
• 通讯作者: 他2名