Study of the methods for visualizing intracellular signaling molecules

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In the present study, we studied the development of optical probes for visualizing bio molecules using luciferases, GFP and its spectral variants based on protein reconstitution methods. Moreover, we generated new concepts for molecular imaging such as luciferase cyclization by protein splicing and spatiotemporal analysis of protein-protein interactions based on split luciferase complementation. These results are expected to contribute to resolving biological function in live cells.