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Effect of Australian cypress essential oils on Anti-obesity and elucidation of the mechanisms

澳大利亚丝柏精油的抗肥胖作用及其机制的阐明

基本信息

批准号：
18380104
负责人：
MITSUNAGA Tohru
金额：
$ 10.58万
依托单位：
Gifu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

项目摘要

Fist of all, we tried to fractionate the Australian cypress eccential oils with liquid-liquid partition method and to analyse that with GC-MS in this study. It turned out that most of the oil partitioned was neutral fraction. We could fractionate an ingredient full of volatility in an oil by the distillation and further a fine separation was successful using silica gel column chromatography. The main constituents of the cypress oil consisted of (-) -citronellic acid as monoterpene and guaiol, α -,β-, Υ-eudesmol as sesquiterpene alcohol.Next we investigated the inhibitory effect of adiposity stimulation by inhalating the essential oil using a mouse and a rat. Body weight of the mouse inhalated 100 times dilution oil increased comparing to the control group. Also, body weight of the mouse inhalated 1,000 times dilution oil decreased, and it became clear that the optimum density of the oil refining was present to show obese inhibitory effect because blood glycerol (GL) level increased. By … More the oil refining inhalation experiment wing a cannulation rat the possibility that resolving power from TG to GL was sublimed into was thought aboutFurthermore, as for the effect, the citronellic acid which decreased cholesterol (TC) and TG of the serum by having given a whiff of guaiol which was the one of the main constituents of the oil refining, it revealed an effect to decrease TC and TG of the liver. When these two constituents might be associated with oil refining inhalational obese inhibitory effect than this greatly. Also, the decrease of blood catecholamines was held in check by the inhalation of the oil refining, and possibility to prevent a resolving power fall of TG in the white adipose tissue was thought about.Furthermore, it was thought that the reason why it became clear by this study that a white cypress oil refining constituents had affinity for vaniloid receptor which recognized capsaicin, and TG decreased by oil refining inhalation was that an oil refining constituents bound to this receptor, and triglyceride resolution action was activated. Less

本研究首先尝试用液-液分配法对澳大利亚柏树挥发油进行分离，并用GC-MS对其进行分析。事实证明，大部分被分配的石油是中性馏分。我们可以通过蒸馏将油中的挥发性成分分离出来，然后用硅胶柱层析成功地进行精细分离。柏油的主要成分为(-)-香茅酸为单萜，愈创木酚为倍半萜醇类，α-，β-，Υ-桉树酚为倍半萜醇。吸入100倍稀释油的小鼠体重较对照组增加。此外，吸入1000倍稀释油的小鼠体重下降，很明显，由于血甘油(GL)水平上升，炼油的最佳浓度存在，以显示抑制肥胖的效果。作者：…此外，还考虑了将甘油三酯分解为甘油三酯升华为甘油三酯的可能性。此外，香茅酸通过吸入炼油的主要成分愈创木酚来降低血清中的胆固醇(TC)和甘油三酯(TG)，显示了降低肝脏TC和TG的作用。当这两种成分可能与炼油有关时，吸入性肥胖的抑制作用比这要大得多。此外，通过吸入炼油可以抑制血儿茶酚胺的下降，并考虑了防止白色脂肪组织中TG的分辨率下降的可能性。此外，本研究进一步认为，白柏炼油成分与识别辣椒素的香草素受体有亲和力，而吸入炼油成分降低TG的原因是炼油成分与该受体结合，激活了甘油三酯的分解作用。较少