Effect of Australian cypress essential oils on Anti-obesity and elucidation of the mechanisms

本研究首先采用液液分离法对澳洲柏树精油进行分离，并采用气相色谱-质谱联用技术对其进行分析。结果表明，分馏出的油大部分为中性馏分。通过蒸馏法分离出油中挥发性较强的成分，并用硅胶柱层析法进行了精细分离。柏树油的主要成分为(-)-香茅烯酸（单萜）和愈创木酚、α -、β-、Υ-eudesmol（倍半萜醇）。接下来，我们用小鼠和大鼠分别吸入精油来研究脂肪刺激的抑制作用。与对照组相比，吸入100倍稀释油的小鼠体重增加。同时，吸入1000倍稀释油的小鼠体重下降，且由于血甘油（GL）水平升高，明显存在抑制肥胖作用的最佳精炼油密度。通过对大鼠进行的精炼吸入实验，进一步探讨了将甘油三酯分解为GL的可能性。此外，在作用方面，香橼酸通过吸入甘油三酯的主要成分之一愈创木酚来降低血清中胆固醇（TC）和TG，显示出降低肝脏TC和TG的作用。当这两种成分可能与炼油相关时，其吸入性肥胖抑制作用比此大得多。此外，血液中儿茶酚胺的减少也因吸入精炼油而得到控制，并考虑了防止白色脂肪组织中TG的分辨能力下降的可能性。此外，本研究明确了白柏树炼油成分与识别辣椒素的香草素受体具有亲和力，吸入炼油后TG降低的原因是炼油成分与该受体结合，激活了甘油三酯的分解作用。少

Fist of all, we tried to fractionate the Australian cypress eccential oils with liquid-liquid partition method and to analyse that with GC-MS in this study. It turned out that most of the oil partitioned was neutral fraction. We could fractionate an ingredient full of volatility in an oil by the distillation and further a fine separation was successful using silica gel column chromatography. The main constituents of the cypress oil consisted of (-) -citronellic acid as monoterpene and guaiol, α -,β-, Υ-eudesmol as sesquiterpene alcohol.Next we investigated the inhibitory effect of adiposity stimulation by inhalating the essential oil using a mouse and a rat. Body weight of the mouse inhalated 100 times dilution oil increased comparing to the control group. Also, body weight of the mouse inhalated 1,000 times dilution oil decreased, and it became clear that the optimum density of the oil refining was present to show obese inhibitory effect because blood glycerol (GL) level increased. By … More the oil refining inhalation experiment wing a cannulation rat the possibility that resolving power from TG to GL was sublimed into was thought aboutFurthermore, as for the effect, the citronellic acid which decreased cholesterol (TC) and TG of the serum by having given a whiff of guaiol which was the one of the main constituents of the oil refining, it revealed an effect to decrease TC and TG of the liver. When these two constituents might be associated with oil refining inhalational obese inhibitory effect than this greatly. Also, the decrease of blood catecholamines was held in check by the inhalation of the oil refining, and possibility to prevent a resolving power fall of TG in the white adipose tissue was thought about.Furthermore, it was thought that the reason why it became clear by this study that a white cypress oil refining constituents had affinity for vaniloid receptor which recognized capsaicin, and TG decreased by oil refining inhalation was that an oil refining constituents bound to this receptor, and triglyceride resolution action was activated. Less