Exhaustive analysis of metabolic substances by olfactory stimulus of cypress essential oil and anti-obese mechanism

吸入柏树精油(CEO)对高脂饮食饲养的小鼠有一定的减肥作用。为了阐明CeO对小鼠肥胖的作用机制，我们用电生理的方法观察了CeO的嗅觉刺激是否兴奋了支配大鼠肩峰间棕色脂肪组织的交感神经。通过液-液分配、制备高效液相色谱和柱色谱分离得到(-)-柠檬酸(CA)、愈创木酚、α，β-，γ-桉叶酚混合组分(GE)、愈创木酚(G)和β-桉树酚组分(E)。测定麻醉大鼠棕色脂肪组织(BSNA)的交感神经活动。用100倍体积的水作为悬浮液对CeO、Ge、G、E进行嗅觉刺激时，BSNA显著升高，而CA无明显变化。上述结果表明，头孢菌素的嗅觉刺激增强了BSNA，其活性成分为愈创木酚和β-桉叶酚。

Inhaling Cypress Essential Oil (CEO) showed anti-obese effect of mice breeding with high fat diet. In order to clarify the mechanism of CEO on anti-obesity of mouse we examined whether the olfactory stimulation with CEO excites sympathetic nerves innervating the interscapular brown adipose tissue in rats by using electrophysiological methods. (-)-Citronellic acid (CA), guaiol, α, β-, γ-eudesmol mixture fraction (GE), guaiol (G) and β-eudesmol fraction (E) were obtained by liquid-liquid partition, preparative HPLC and column chromatography from CEO. The sympathetic nerve activity on brown adipose tissue (BSNA) was measured in anesthetized rat. When olfactory stimulations with CEO, GE, G, E suspended in 100 volumes of water were performed, the BSNA significantly elevated respectively, but CA has no changes. These results show that olfactory stimulation with CEO enhances BSNA and the active ingredients were guaiol and β-eudesmol.