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Identification of risk factors related to poor angiogenic potency of bone marrow cells from different patients

鉴定与不同患者的骨髓细胞血管生成能力差相关的危险因素

基本信息

批准号：
18390378
负责人：
HAMANO Kimikazu
金额：
$ 10.86万
依托单位：
Yamaguchi University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18390378/
关键词：
Ischemia Bone marrow cells Therapeutic Angiogenesis Risk factor

项目摘要

Therapeutic angiogenesis induced by the implantation of autologous bone marrow-derived cells has been iced for the treatment of ischemic diseases. However, clinical trials have shown obvious individual differences in the improvement of regional perfusion after the implantation of autologous bone marrow cells. In this study, we aimed to identify the risk factors related to the functional impairment of bone marrow cells for inducing therapeutic angiogenesis.We collected clinical and laboratory data from 25 patients scheduled to undergo sternotomy for various surgical procedures. Then, we aspirated bone marrow cells from the sternum during the operation, and investigated their angiogenic potency in vitro by cultivation, and in vivo using an ischemic limb model in SCID mice. We identified that aging, renal failure, anemia, and high serum levels of triglyceride, CRP, IL-6, and NTX (the markers of bone metabolism) were significantly correlated with poor angiogenic potency of bone marrow cells. We assigned scores to these risk factors according to their Pvalue, and found a strung correlation between the risk scores of patients and the angiogenic potency of their bone marrow cells (r=0.778, P<0.001). In elusion, we identified the risk factors related to poor angiogenic potency of bone marrow cells. Our results provide important information to help us select patients who will benefit most from this treatment in future clinical trials.

自体骨髓源性细胞植入诱导的治疗性血管生成已被应用于缺血性疾病的治疗。然而，临床试验显示，自体骨髓细胞移植后局部灌注的改善存在明显的个体差异。在这项研究中，我们旨在确定与骨髓细胞功能损伤相关的危险因素，以诱导治疗性血管生成。我们收集了25例胸骨切开术患者的临床和实验室资料。然后，我们在手术过程中从胸骨抽取骨髓细胞，通过体外培养和体内缺血肢体模型研究其血管生成能力。我们发现，衰老、肾功能衰竭、贫血、血清中甘油三酯、CRP、IL-6和NTX（骨代谢的标志物）的高水平与骨髓细胞血管生成能力差显著相关。我们根据这些风险因素的P值对其进行评分，发现患者的风险评分与其骨髓细胞的血管生成能力之间存在串相关（r=0.778， P<0.001）。最后，我们确定了与骨髓细胞血管生成能力差相关的危险因素。我们的结果提供了重要的信息，帮助我们在未来的临床试验中选择最受益于这种治疗的患者。