Identification of risk factors related to poor angiogenic potency of bone marrow cells from different patients

自体骨髓源性细胞移植诱导的治疗性血管生成已被用于治疗缺血性疾病。然而，临床试验显示，自体骨髓细胞植入后局部灌注的改善存在明显的个体差异。在这项研究中，我们的目的是确定相关的风险因素，骨髓细胞诱导治疗性血管生成的功能障碍，我们收集了25例患者的临床和实验室数据，计划接受胸骨切开术的各种外科手术。然后，我们在手术过程中从胸骨抽吸骨髓细胞，并通过培养和在SCID小鼠中使用缺血肢体模型在体内研究其血管生成潜能。我们发现，衰老、肾功能衰竭、贫血和高血清甘油三酯、CRP、IL-6和NTX（骨代谢标志物）水平与骨髓细胞血管生成能力差显著相关。根据P值对这些危险因素进行评分，发现患者的危险评分与其骨髓细胞的血管生成能力呈强相关（r=0.778，P<0.001）。在实验中，我们确定了与骨髓细胞血管生成能力差相关的风险因素。我们的研究结果提供了重要的信息，帮助我们选择在未来的临床试验中从这种治疗中获益最多的患者。

Therapeutic angiogenesis induced by the implantation of autologous bone marrow-derived cells has been iced for the treatment of ischemic diseases. However, clinical trials have shown obvious individual differences in the improvement of regional perfusion after the implantation of autologous bone marrow cells. In this study, we aimed to identify the risk factors related to the functional impairment of bone marrow cells for inducing therapeutic angiogenesis.We collected clinical and laboratory data from 25 patients scheduled to undergo sternotomy for various surgical procedures. Then, we aspirated bone marrow cells from the sternum during the operation, and investigated their angiogenic potency in vitro by cultivation, and in vivo using an ischemic limb model in SCID mice. We identified that aging, renal failure, anemia, and high serum levels of triglyceride, CRP, IL-6, and NTX (the markers of bone metabolism) were significantly correlated with poor angiogenic potency of bone marrow cells. We assigned scores to these risk factors according to their Pvalue, and found a strung correlation between the risk scores of patients and the angiogenic potency of their bone marrow cells (r=0.778, P<0.001). In elusion, we identified the risk factors related to poor angiogenic potency of bone marrow cells. Our results provide important information to help us select patients who will benefit most from this treatment in future clinical trials.