NEW TREATMENT FOR MYOCARDIAL INFARCTION BY XENO-FETAL CARDIOMYOCYTE TRANSPLANTATION

异种胎儿心肌细胞移植治疗心肌梗死的新方法

基本信息

  • 批准号:
    11671326
  • 负责人:
  • 金额:
    $ 2.43万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    1999
  • 资助国家:
    日本
  • 起止时间:
    1999 至 2000
  • 项目状态:
    已结题

项目摘要

Background. Experimental allografting of fetal cardiomyocytes has been performed successfully. In this study, we attempted to transplant rat fetal cardiomyocytes into the hearts of mice by blocking the CD28/B7 co-stimulatory pathway via CTLA4-Ig gene transfer.Methods. Fetal cardiomyocytes derived from Dark Agouti (DA) rat were infected with CTLA4-Ig-expressing adenovirus vectors (AdCTLA) and injected directly into the normal myocardium of C3H/He mice (n=15). For control, cells infected with β-Gal-expressing adenovirus vector (AdRL) and cells without infection were injected into additional mice (n=30). The percentages of cardiomyocytes infection by AdRL or AdCTLA were estimated by X-gal staining or immunostaining with anti-CTLA4-Ig antibody. Mice were killed at 2 (n=5), 4 (n=5), and 8 (n=5) weeks after xenotransplantation. Transplanted fetal cardiomyocytes were examined for survival by immunostaining with anti-rat artial natriuretic peptide (ANP) and anti-CTLA4-Ig antibodies.Results. X-gal staining and immunostaining showed infection to be approximately 30% at 10 MOI, 65% at MOI 50, and 50〜70% at MOI>100, 3 days after infection with AdCTLA or AdRL.The cardiomyocytes infected with AdCTLA expressed CTLA4-Ig and survived to 8 weeks after xenotransplantation in all of these mice. However, cardiomyocytes infected with AdRL and non-infected cells were not detected even 2 weeks after xenotransplantation.Conclusion. Fetal cardiomyocytes were successfully infected by AdCTLA and AdRL.Survival of xenografted fetal cardiomyocytes is prolonged by adenovirus-mediated CTLA4-Ig expression.
背景胚胎心肌细胞的实验性同种异体移植已成功进行。本研究通过转CTLA 4-IG基因阻断CD 28/B7共刺激通路,将大鼠胚胎心肌细胞移植到小鼠心脏中。用表达CTLA 4-Ig的腺病毒载体(AdCTLA)感染来自Dark Agglutination(DA)大鼠的胎儿心肌细胞,并将其直接注射到C3 H/He小鼠(n=15)的正常心肌中。对于对照,将用β-Gal表达腺病毒载体(AdRL)感染的细胞和未感染的细胞注射到另外的小鼠(n=30)中。用X-gal染色或抗CTLA 4-IG抗体免疫组化法检测AdRL或AdCTLA感染心肌细胞的百分率。在异种移植后2(n=5)、4(n=5)和8(n=5)周处死小鼠。用抗大鼠心钠素(ANP)和抗CTLA 4-IG抗体免疫组化染色检测移植的胎儿心肌细胞存活情况。X-gal染色和免疫组化结果显示,感染后第3天,感染率在10 MOI时为30%,在MOI 50时为65%,在MOI>100时为50 ~ 70%,并表达CTLA 4-IG。然而,即使在异种移植后2周,AdRL感染的心肌细胞和未感染的心肌细胞也未被检测到。用AdCTLA和AdRL成功感染胎鼠心肌细胞,腺病毒介导的CTLA 4-IG表达可延长胎鼠心肌细胞移植存活时间。

项目成果

期刊论文数量(6)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Niimi M: "Oral delivery of xeno-antigen combined with non-depleting anti-CD4 monoclonal antibody induces significantly prolonged survival of concordant skin xenograft."Xenotransplantation. 8. 75-79 (2001)
Niimi M:“口服异种抗原与非消耗性抗 CD4 单克隆抗体相结合,可显着延长一致皮肤异种移植物的存活时间。”异种移植。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Niimi M, et al.: "Oral delivery of xeno-antigen combined with non-depleting anti-CD4 monoclonal antibody induces significantly prolonged survival of concordant skinxenograft."Xenotransplantation. 8. 75-79 (2001)
Niimi M 等人:“口服异种抗原与非消耗性抗 CD4 单克隆抗体相结合,可显着延长一致皮肤异种移植物的存活时间。” 异种移植。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Hamano K: "Graft vasculopathy and tolerance : does the balance of Th cells contribute to graft vasculopathy?"J Surg Res. 93. 28-34 (2000)
Hamano K:“移植血管病变和耐受性:Th 细胞的平衡是否会导致移植血管病变?”J Surg Res。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Hamano K, et al.: "Graft vasculopathy and tolerance : does the balance of Th cells contribute to graft vasculopathy?"Journal of Surgical Research. 93. 28-34 (2000)
Hamano K 等人:“移植血管病变和耐受性:Th 细胞的平衡是否会导致移植血管病变?”《外科研究杂志》。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Niimi M, et al.: "Oral delivery of xeno-antigen combined with non-depleting anti-CD4 monoclonal antibody induces significantly prolonged survival of concordant skin xenograft."Xenotransplantation. 8. 75-79 (2001)
Niimi M 等人:“口服异种抗原与非消耗性抗 CD4 单克隆抗体相结合,可显着延长一致皮肤异种移植物的存活时间。”异种移植。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

HAMANO Kimikazu其他文献

HAMANO Kimikazu的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('HAMANO Kimikazu', 18)}}的其他基金

Development of exosomes accumulating in ischemic tissues for angiogenesis
外泌体在缺血组织中积累以促进血管生成的发展
  • 批准号:
    19K22660
  • 财政年份:
    2019
  • 资助金额:
    $ 2.43万
  • 项目类别:
    Grant-in-Aid for Challenging Research (Exploratory)
Development of vascular regeneration therapy by exosome derived from enhanced cells
利用增强细胞衍生的外泌体开发血管再生疗法
  • 批准号:
    19H03739
  • 财政年份:
    2019
  • 资助金额:
    $ 2.43万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Challenges to rejuvenate aged-cardiac stem cells by genome editing to develop next generation therapeutic strategies for heart failure.
通过基因组编辑使衰老的心脏干细胞恢复活力以开发下一代心力衰竭治疗策略的挑战。
  • 批准号:
    15K15508
  • 财政年份:
    2015
  • 资助金额:
    $ 2.43万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Development of transplantation therapy using hypoxically preconditioned cell sheets for lower limb ischemic ulcers
低氧预处理细胞片移植治疗下肢缺血性溃疡的进展
  • 批准号:
    15H04939
  • 财政年份:
    2015
  • 资助金额:
    $ 2.43万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Challenges to identify endogenous factors associating with cardiac regeneration in heart failure.
确定与心力衰竭心脏再生相关的内源性因素面临的挑战。
  • 批准号:
    23659673
  • 财政年份:
    2011
  • 资助金额:
    $ 2.43万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Novel therapeutic strategies using autologous bone marrow-derived stem cells for vascular regeneration
使用自体骨髓干细胞进行血管再生的新治疗策略
  • 批准号:
    23390336
  • 财政年份:
    2011
  • 资助金额:
    $ 2.43万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Identification of Risk Factors Related to Poor Angiogenic Potential of Bone Marrow Cells from Different Patients
鉴定与不同患者的骨髓细胞血管生成潜能差相关的危险因素
  • 批准号:
    20390370
  • 财政年份:
    2008
  • 资助金额:
    $ 2.43万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Identification of risk factors related to poor angiogenic potency of bone marrow cells from different patients
鉴定与不同患者的骨髓细胞血管生成能力差相关的危险因素
  • 批准号:
    18390378
  • 财政年份:
    2006
  • 资助金额:
    $ 2.43万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Development of tailor-made regenerative therapy for heart failure by using autologous bone marrow stem cells
利用自体骨髓干细胞开发针对心力衰竭的定制再生疗法
  • 批准号:
    16390397
  • 财政年份:
    2004
  • 资助金额:
    $ 2.43万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
THERAPEUTIC ANGIOGENESIS INDUCED BY AUTOLOGOUS BONE MARROW CELLS IMPLANTATION FOR THE TREATMENT OF ISCHEMIC DISEASES
自体骨髓细胞植入诱导治疗性血管生成治疗缺血性疾病
  • 批准号:
    13671391
  • 财政年份:
    2001
  • 资助金额:
    $ 2.43万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了