Novel therapeutic strategies using autologous bone marrow-derived stem cells for vascular regeneration

点击翻译按钮获取中文摘要

Autologous transplantation of stem cells often shows limited clinical outcomes, and a discrepancy of therapeutic effects between patients have been reported. Based on our previsous studies demonstrating that hypoxic pre-treatment reinforces cellular functions in small animal experiments, we planned to appply hypoxic pre-treatment protocol to patients with vascular disease. In this study, we examined wether autologous transplantation of peripheral blood-derived stem cells (PBSCs) which have preconditioned in hypoxic incubation accelerates anigogenesis in the hypixic tissues. Autologous transplantation of preconditioned-PBSCs resulted in an acceleration of angiogenesis and an improvement of blood flow in the hypoxia-induced hindlimb of middle animals. We also demonstrated that hypoxic pretreatment induces reinforcement of cellular functions in human PBSCs. Taken together, hypoxic pretreatment of stem cells is promising therapeutic approach for human clinical trials.