Study of cell regulation by sodium sensor channels

钠传感器通道的细胞调节研究

• 批准号: 18500251
• 负责人: WATANABE Eiji
• 金额: $ 2.57万
• 依托单位: National Institute for Basic Biology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18500251/
• 关键词: glia; sodium; sensor; homeostasis; channel; circumventricular organs; gene-targetine mouse; cell culture

Sodium homeostasis is crucial for life and Na-levels in body fluids are constantly monitored in the brain. The subfornical organ (SFO) is the center of the sensing responsible for the control of Na-intake behavior, where Nax channels are expressed in specific glial cells as the sodium-level sensor. Here, we show direct interaction between Nax channels and subunits of Na+/K+-ATPase, which brings about Na-dependent activation of the metabolic state of the glial cells. The metabolic enhancement leading to extensive lactate production was observed in the SFO of wild-type mice, but not of the Nax-knockout mice. Furthermore, lactate, as well as Na, stimulated the activity of GABAergic neurons in the SFO. These results suggest that the information on a physiological increase of the Na level in body fluids sensed by Nax in glial cells is transmitted to neurons by lactate as a mediator to regulate neural activities of the SFO.

钠稳态对生命至关重要，体液中的钠水平在大脑中不断受到监测。穹隆下器(SFO)是控制钠摄取行为的感觉中枢，NAx通道作为钠水平传感器在特定的神经胶质细胞中表达。在这里，我们展示了Nax通道和Na+/K+-ATPase亚单位之间的直接相互作用，这导致了神经胶质细胞代谢状态的钠依赖激活。在野生型小鼠的SFO中观察到了导致广泛乳酸产生的代谢增强，但在NAX基因敲除小鼠中没有观察到。此外，乳酸和Na也能刺激SFO内GABA能神经元的活动。这些结果表明，神经胶质细胞中Nax感受到的体液钠水平生理性升高的信息是通过乳酸作为媒介传递到神经元，以调节SFO的神经活动。

项目成果

脳のナトリウムレベルセンサー〜遺伝子改変マウスによる解析〜

脑钠水平传感器~使用转基因小鼠进行分析~

• 发表时间: 2007
• 作者: Shimizu;H.;Watanabe;E.;Hiyama;T.Y.;Nagakura;A.;Fujikawa;A.;Noda;M.;Ayano Nagakura;Hidetada Shimizu;渡辺 英治
• 通讯作者: 渡辺 英治

Before and After sodium sensor

钠传感器之前和之后

• 发表时间: 2008
• 作者: Watanabe;E.
• 通讯作者: E.

Sodium-level sensor of the brain

大脑的钠水平传感器

• 发表时间: 2007
• 作者: Watanabe;E.
• 通讯作者: E.

脳のナトリウムセンサー〜グリアは脳の情報処理解明のブレイクスルーに成り得るか?〜

大脑中的钠传感器：神经胶质细胞能否成为阐明大脑信息处理的突破？

• 发表时间: 2007
• 作者: Watanabe;E.;渡辺 英治
• 通讯作者: 渡辺 英治

Characterization of neurons in the mouse subfbrnical organ by retrograde labeling

通过逆行标记表征小鼠毛下器官中的神经元

• 发表时间: 2007
• 作者: Shimizu;H.;Watanabe;E.;Hiyama;T.Y.;Nagakura;A.;Fujikawa;A.;Noda;M.;Ayano Nagakura
• 通讯作者: Ayano Nagakura