Elucidation of the biosynthesis and the biological function of DKxanthenes during fruiting body formation in Myxococcus xanthus

阐明黄粘球菌子实体形成过程中 DKxanthenes 的生物合成和生物学功能

• 批准号: 53572187
• 负责人: Professor Dr. Rolf Müller
• 金额: --
• 依托单位: Helmholtz-Institut für Pharmazeutische Forschung Saarland
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2009-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/53572187?language=en
• 关键词: Elucidation; biosynthesis; biological; function; DKxanthenes

This project aims to investigate the role of a recently discovered novel class of secondary metabolites - the DKxanthenes - during fruiting body formation by the myxobacterial model organism Myxococcus xanthus. Under starvation conditions, cells enter the developmental life cycle which culminates in the differentiation of vegetative cells into stress-resistant myxospores within the fruiting body. Mutants unable to produce the DKxanthene pigments were shown to be strongly delayed in mature spore production. The underlying mechanism remains unclear, even though the lipid-like structure and the assigned antioxidative capacity of the DKxanthenes imparts a protective effect, most likely within the cell membrane. In order to understand the physiological processes underlying the absence and/or presence of the DKxanthenes, this work aims to analyze DKxanthene biosynthesis on the molecular biological level, as well as to determine the differing morphological and stress-resistance properties of M. xanthus wild type and DKxanthene-negative cells.

本项目旨在研究最近发现的一类新的次生代谢物--DKxanthenes--在粘细菌模式生物--黄色粘球菌子实体形成过程中的作用。在饥饿条件下，细胞进入发育的生命周期，最终在子实体内将营养细胞分化为抗逆境的粘孢子。不能产生DKxanthene色素的突变株在成熟孢子生产中表现出强烈的延迟。其潜在的机制尚不清楚，尽管DKxanthenes的类脂结构和指定的抗氧化能力提供了保护作用，很可能是在细胞膜内。为了了解DKxanthenes缺失和/或存在的生理过程，本工作旨在从分子生物学水平上分析DKxanthene的生物合成，并确定野生型和DKxanthene阴性细胞的不同形态和抗逆性。