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Study on role of motor proteins in intracellular transport of pituitary hormone

Study on role of motor proteins in intracellular transport of pituitary hormone
运动蛋白在垂体激素细胞内转运中的作用研究
批准号:
18591608
负责人:
MATSUNO Akira
金额:
$2.4万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2006
资助国家:
日本
项目状态:
已结题
起止时间:
2006 至 2007

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中文摘要
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英文摘要
We focused on kinesin superfamily (KIF) among motor proteins related with the intracellular transport of pituitary hormone.The identification of KIF working synergistically with rab 3B, which is an important molecule in growth hormone (GH) transport, was carried out.We synthesized fusion protein of KIF and green fluorescent protein (GFP). EGFP-KIF and ECFP-Rab3B were co-transfected in GH3 cell and AtT-20 cell that express EYFP-GH.Moreover, the suppression of KIF using siRNA was examined.We confirmed the suppression of KIF led to the inhibition of the secretion of GH.The results of these experiments were observed under confocal laser scanning microscopy.
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会议论文
Correlation among K1-67 staining index, expression of somatostatin receptor, growth hormone dynamics in growth hormone releasing hormone, bromocriptine, or somatostatin loading test in patients with pituitary somatotroph adenoma
垂体生长激素腺瘤患者生长激素释放激素、溴隐亭或生长抑素负荷试验中K1-67染色指数与生长抑素受体表达、生长激素动态的相关性
DOI: --
发表时间: 2006
期刊: Growth hormone & IGF research 16[Suppl.B]
影响因子: --
作者: [Matsuno A, et al.]
通讯作者: et al.
DOI: --
发表时间: 2006
期刊: Brain Tumor Pathology 23
影响因子: --
作者: [Matsuno A, et al.]
通讯作者: et al.
R-Ras regulates exocytosis by Rgl2/Rlf-mediated activation of RalA on endosomes
R-Ras 通过 Rgl2/Rlf 介导的内体上 RalA 激活调节胞吐作用
DOI: --
发表时间: 2007
期刊: Mol. Biol. Cell
影响因子: --
作者: [Takaya A, Kamio A, Masuda M, Mochizuki N, Sawa H, Sato M, Nagashima K, Mizutani A, Matsuno A, Kiyokawa E, Matsuda M]
通讯作者: Matsuda M
An insight of subcellular localization of pituitary hormone mRNA : observation under electron microscopy and confocal laser scanning microscopy
垂体激素 mRNA 亚细胞定位的见解:电子显微镜和共焦激光扫描显微镜下的观察
DOI: --
发表时间: 2007
期刊: New Messenger RNA Research Communications, Nova Science Publishers, Inc. Matsuno
影响因子: --
作者: [A, Itoh J, Utsunomiya, H, Takekoshi, S, Osamura, RY]
通讯作者: RY
12
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      20591723
    • 项目类别:
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    • 资助金额:
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    • 财政年份:
      2008
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    • 项目类别:
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    • 资助金额:
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      1997
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    • 项目类别:
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