Studies on a novel gene therapy of glioma using antisense oligonucletide for microtubule-associated protein mRNA

点击翻译按钮获取中文摘要

Microtubule-associated protein (MAP) lA, one of the well-known MAPs that are essential for the various bioactivities, is known to be expressed in cultured glioma cells such as rat C6 glioma cells. The antisense oligodeoxynucleotide for MAP lA is demonstrated, by colony forming assay, to have significantly suppressed the in vitro proliferation of rat 6 glioma cells. The effect of antisense oligodeoxynucleotide on the expression of MAP lA has been demonstrated by immunohistochemical staining. This report will be the first one describing the efficiency of antisense oligodeoxynucleotide for MAP lA on the growth suppression of rat C6 glioma cells. The effect of antisense oligodeoxynucleotide for MAP 1A on the cell cycle of rat C6 glioma cells has also been shown by the flow cytometrical analysis. The suppression of MAP IA resulted in GL arrest of C6 glioma cells, and this result strongly suggests the important role of MAP IA in the cell cycle. The suppression of MAP lA using antisense oligodeoxynudeotide strategy can be established as a novel antitumor therapy for gliomas.