Study on the endocrine function and cytogenesis of pituitary adenomas

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The expression of pituitary hormone mRNAs in 17 somatotroph adenomas, 4 lactotroph adenomas, and 14 clinically non-functioning adenomas was examined with the aid of non-isotopic ISH and Northern bolt analysis. ISH and Northern blot hybridization studies of lactotroph adenomas revealed their hormonal gene expression confined to PRL.They are considered to be derived from well differentiated lactotroph cells. ISH study of 17 somatotroph adenomas revealed plurihormonal gene expression such as PRL(13/17), ACTH(8/17), FSHbeta(5/17), LHbeta(3/17), alpha-subunit(4/17), TSHbeta(3/17). In some cases whose pituitary hormones were immunohistochemically negative, their mRNAs, mainly ACTH, FSHbeta, LHbeta, mRNAs, were detected by ISH.It is suggested that some somatotroph adenomas, which were considered to originate from acidophil cell lines, might be derived from plurihormonal primordial stem cells. ISH study of 14 clinically non-functiong adenomas revealed gene expression of GH(4/14), PRL(5/14), ACTH(5/14) in addition to FSHbeta(5/14), LHbeta(4/14), alpha-subunit(1/14). These results suggest that some clinically non-functiong adenomas, which were considered to be subtype of gonadotroph adenoma, might be derived from plurihormonal primordial stem cells.