The study for the regenerative med nc of articular cartilage using cell sheet techonology

一些治疗关节软骨全层缺损的方法，如培养软骨细胞移植，已经完成。然而，为了克服骨关节炎，我们必须进一步研究关节软骨的部分厚度缺陷。修复部分厚度缺陷要困难得多，因为很少有修复细胞能处理这样的损伤部位。我们在此表明，使用温度响应培养皿的生物工程层状软骨细胞片可能是治疗部分厚度缺陷的潜在有用方法。我们使用实时PCR和组织学结果评估这些薄片的性质，并将这些薄片移植到兔部分模型中以评估治疗效果。综上所述，层状软骨细胞片能够维持软骨表型，并且可以附着在软骨损伤的部位，并作为屏障防止这些部位的蛋白聚糖损失，并保护它们免受关节分解代谢因子的影响。

Some treatments for full thickness defects of articular cartilage, such as cultured chondrocytes transplantation, have already been done. However, to overcome osteoarthritis, we must further study the partial thickness defect of articular cartilage. It is much more difficult to repair a partial thickness defect because few repairing cells can address such injured sites. We herein show that bioengineered layered chondrocyte sheets using emperature-responsive culture dishes may be a potentially useful treatment for partial thickness defects. We evaluated the property of these sheets using real-time PCR and histological findings, and allografted these sheets to evaluate the effect of treatment using a rabbit partial model. In conclusion, layered chondrocyte sheets were able to maintain the cartilageous phenotype, and could be attached to the sites of cartilage damage and which acted as a barrier to prevent a loss of proteoglycan from these sites and to protect them from catabolic factors in the joint.