The study for the regenerative med nc of articular cartilage using cell sheet techonology

细胞片层技术对关节软骨再生医学的研究

• 批准号: 18591644
• 负责人: SATO Masato
• 金额: $ 2.41万
• 依托单位: Tokai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18591644/
• 关键词: Temperature-responsive culture dish; Cell sheet; Tissue Engineering; Chondrocyte; Partial thickness defect; Articular cartilage; 軟骨再生; 軟骨修復

Some treatments for full thickness defects of articular cartilage, such as cultured chondrocytes transplantation, have already been done. However, to overcome osteoarthritis, we must further study the partial thickness defect of articular cartilage. It is much more difficult to repair a partial thickness defect because few repairing cells can address such injured sites. We herein show that bioengineered layered chondrocyte sheets using emperature-responsive culture dishes may be a potentially useful treatment for partial thickness defects. We evaluated the property of these sheets using real-time PCR and histological findings, and allografted these sheets to evaluate the effect of treatment using a rabbit partial model. In conclusion, layered chondrocyte sheets were able to maintain the cartilageous phenotype, and could be attached to the sites of cartilage damage and which acted as a barrier to prevent a loss of proteoglycan from these sites and to protect them from catabolic factors in the joint.

目前已有一些治疗关节软骨全层缺损的方法，如培养软骨细胞移植等。然而，要克服骨性关节炎，必须进一步研究关节软骨的部分厚度缺陷。修复部分厚度缺陷要困难得多，因为很少有修复细胞能够修复这样的损伤部位。我们在这里表明，使用温度响应型培养皿的生物工程分层软骨细胞片可能是一种潜在的有用的治疗部分厚度缺陷的方法。我们使用实时定量聚合酶链式反应和组织学结果评估了这些薄片的性能，并使用兔部分模型将这些薄片移植以评估治疗效果。综上所述，层状软骨细胞片能够保持软骨的表型，可以附着在软骨损伤部位，起到屏障的作用，防止这些部位的蛋白多糖丢失，并保护它们免受关节内分解代谢因素的影响。

项目成果

Cell Sheet Technoogy for Cartilage Repair

软骨修复细胞片技术

• 发表时间: 2006
• 作者: Sato M;et. al.
• 通讯作者: et. al.

Bioengineered chondrocyte sheets may be potentially useful for the treatment of partial thickness defects of articular cartilage

• DOI: 10.1016/j.bbrc.2006.08.096
• 发表时间: 2006-10-20
• 期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
• 影响因子: 3.1
• 作者: Kaneshiro, Nagatoshi;Sato, Masato;Mochida, Joji
• 通讯作者: Mochida, Joji