The repairing effect of articular cartilage by cellular spheroids composed ofsynovial cells and chondrocytes

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Autologous Chondrocyte Transplantation(ACT) has been chosen as the treatment for Osteoarthritis. However, this technique needs the articular chondrocytes(AC) from healthy cartilage, which associates donor site morbidity. As AC from aged patents show limited proliferation in vitro, ACT is not accepted widely for treatment of ostearthritic lesions. On the other hand, the synovium derived cells(SY) whose high proliferation potency does not changed regardless of age have been focusing the localization mesenchymal stem cells of differentiation potency into chondrocytes. AC and SY from 4 knee of a Japanese white rabbit were isolated with 2 step enzymatic digestion and cultured, the passage growth rate of the cells were compared. When AC and SY cultured it in a high density suspension state in non-adhesive culture plate, cellular spheroids were collected and then histological evaluated was conducted with toluidine blue, safranin O and immunohistochemistry of collagen type 1 and 2.The gene expressions concerning choncrocyte-specific differentiation were also tested by Reverse Transcriptase-Polymerase Chain Reaction. In results, we successfully prepared a large quantity of cellular spheroids in a short period using mixed AC and SY regardless of cellular component rates. Effective usage of SY is expected as a replacement of AC in ACT.