USE OF PIN1 AS A MOLECULAR PROBE TO IDENTIRY PROSTATE CANCER-SPECIFIC BIOMARKERS

使用 PIN1 作为识别前列腺癌特异性生物标志物的分子探针

• 批准号: 18591765
• 负责人: RYO Akihide
• 金额: $ 2.6万
• 依托单位: Yokohama City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18591765/
• 关键词: PROSTATE CANCER; PHOSPHORYLATION; ENZYME; PROTEIN; ULOROGY; タンパク質

The peptidyl prolyl-isomerase Pinl specifically binds phosphorylated serine or threonine residues immediately preceding proline (pSer/Thr-Pro) in a subset of proteins, and promotes the cis/trans isomerization of the peptide bond. Although Pin 1 has been shown to be involved in cell transformation and maintenance of malignant phenotype in prostate cancer cells (PCa), its specific substrates for malignant transformation remain largely determined. In this study, we utilized Pin 1 as a molecular probe to capture cancer-specific phosphorylated proteins. The glutathione-S-transferase (GST) pull-down and subsequent proteomic analyses newly identified 20 Pca-specific Pin 1 binding proteins(PINBP1-20). We further confirmed up-regulation of these genes in prostate cancer cell lines and tissues. Tissue micro-dissection based quantitative RT-PCR analysis of prostate cancer tissues revealed that the expression of PINBP1 tightly paralleled a higher probability and a shorter period of tumor recurrence following the operation. These results indicate that the Pinl -proteomics analysis can provide a useful tool to identify cancer-specific phosphorylated proteins, which could be potential prognostic marker or anti-cancer targets.

肽基脯氨酰异构酶 Pinl 特异性结合蛋白质子集中脯氨酸 (pSer/Thr-Pro) 之前的磷酸化丝氨酸或苏氨酸残基，并促进肽键的顺式/反式异构化。尽管 Pin 1 已被证明参与前列腺癌细胞 (PCa) 的细胞转化和恶性表型的维持，但其恶性转化的具体底物在很大程度上仍处于确定状态。在这项研究中，我们利用 Pin 1 作为分子探针来捕获癌症特异性磷酸化蛋白。谷胱甘肽-S-转移酶 (GST) 下拉和随后的蛋白质组学分析新鉴定了 20 个 Pca 特异性 Pin 1 结合蛋白 (PINBP1-20)。我们进一步证实了这些基因在前列腺癌细胞系和组织中的上调。基于组织显微解剖的前列腺癌组织定量 RT-PCR 分析显示，PINBP1 的表达与手术后肿瘤复发的概率较高和周期较短密切相关。这些结果表明，Pinl-蛋白质组学分析可以提供一种有用的工具来识别癌症特异性磷酸化蛋白，这可能是潜在的预后标志物或抗癌靶标。

项目成果

An immunohistochemical scoring system of prolyl isomerase Pin1 for predicting relapse of prostate carcinoma after radical prostatectomy

• DOI: 10.1016/j.prp.2005.12.007
• 发表时间: 2006-01-01
• 期刊: PATHOLOGY RESEARCH AND PRACTICE
• 影响因子: 2.8
• 作者: Sasaki, Takeshi;Ryo, Akihide;Aoki, Ichiro
• 通讯作者: Aoki, Ichiro

SOCS1 is an inducible host factor during HIV-1 infection and regulates the intracellular trafficking and stability of HIV-1 Gag

• DOI: 10.1073/pnas.0704831105
• 发表时间: 2008-01-08
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Ryo, Akihide;Tsurutan, Naomi;Yamamoto, Naoki
• 通讯作者: Yamamoto, Naoki

An immunohistochemical scoring system of prolyl isomerase Pinl for predicting relapse of prostate carcinoma after radical prostatectomy

脯氨酰异构酶 Pinl 免疫组织化学评分系统预测前列腺癌根治术后复发

• 发表时间: 2006
• 期刊: Pathol Res Pract 202(5)
• 作者: Sasaki;T;et. al.
• 通讯作者: et. al.

Pin1 is a novel regulator of centrosome duplication

Pin1 是中心体复制的新型调节因子

• 发表时间: 2006
• 期刊: Mol Cell Biol. 26(4)
• 作者: Suizu F;et. al.
• 通讯作者: et. al.

前立腺癌の予後判定方法及びそのための診断薬

确定前列腺癌预后的方法及其诊断剂

• 发表时间: 2006