Generation of monoclonal antibodies targeting induced cancer stem cells

为了获得针对肿瘤干细胞的单抗，我们用诱导的肿瘤干细胞(ICSC)免疫大鼠，然后生产杂交瘤。利用ICSC和乳腺癌细胞株，筛选出两株只识别ICSC而不识别乳腺癌细胞的克隆。蛋白质阵列分析表明，ABL1可能是单抗的靶点。当iCSCs被ABL抑制剂伊马替尼处理时，iCSCs的自我更新特性被抑制。

To obtain monoclonal antibodies specifically targeting cancer stem cells, we immunized rat with induced cancer stem cells (iCSC) followed by the production of hybridomas. By using iCSC and breast cancer cell lines, we selected two monoclon al colones that only recognize iCSC but not brest cancer cell line. Protein array analysis revealed that ABL1 could be a target of the monoclonal antibodies. When iCSCs were treated with an ABL inhibitor, imatinib, self-renewal property of iCSCs was inhibited.