The roles of tenascin-C for corneal epithelial stem cell niche

Tenascin-C 对角膜上皮干细胞生态位的作用

• 批准号: 18591930
• 负责人: MASUDA Akira
• 金额: $ 2.57万
• 依托单位: Kyoto Prefectural University of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18591930/
• 关键词: tenascin-C; stem cell niche; beta catenin; 発現制御; 発生・分化

Immunohistological analysis of ocular stem cell deficiency diseases revealed massive subconjunctival Tenascin-C (TNC) deposition. TNC reporter gene analysis showed TNC gene induction upon wound healing and mechanical stress. The addition of TNC into the fibronectin (FN) coated dishes induced the change of cell shapes. The TNC knockout mouse analysis showed higher level of beta-catenin in the wild type corneal epithelium in the basal membrane side whereas diffuse beta-catenin expression was observed in TNC knockout mouse. The TNC molecule may contribute to the formation of corneal epithelial stem cell homeostasis through controlling beta-catenin signals.

眼部干细胞缺乏性疾病的免疫组织学分析显示大量结膜下Tenascin-C（TNC）沉积。TNC报告基因分析表明，TNC基因诱导伤口愈合和机械应力。在纤维连接蛋白（FN）包被的培养皿中加入TNC可引起细胞形态的改变。TNC敲除小鼠分析显示，在基底膜侧的野生型角膜上皮中β-连环蛋白水平较高，而在TNC敲除小鼠中观察到弥漫性β-连环蛋白表达。TNC分子可能通过控制β-连环蛋白信号参与角膜上皮干细胞稳态的形成。

项目成果

LOXL1 genetic polymorphisms are associated with exfoliation glaucoma in Japanese.

LOXL1 基因多态性与日本人的剥脱性青光眼有关。

• 期刊: Molecular Vision (in press)
• 作者: Mori;K;Imai;K;Matsuda;A;Ikeda;Y;Naruse;S;Hitora-Takeshita;H;Nakano M;Taniguchi;T;Omi;N;Tashiro;K;Kinoshita S;Matsuda A.;Mori K.
• 通讯作者: Mori K.

High-affinity IgE recept or -B chain expression in human mast cells

人肥大细胞中高亲和力 IgE 受体或 -B 链表达

• 期刊: J Immunol Methods In press
• 作者: Matsuda;A;Okayama;Y;Ebihara;N;Yokoi;N;Gao;P;Hamuro;J;Hopkin;JM;Kinoshita;S
• 通讯作者: S

Genetic polymorphisms in the promoter of the interferon gamma receptorl gene are associated with atopic cataracts

干扰素γ受体1基因启动子的遗传多态性与特应性白内障有关

• 发表时间: 2007
• 期刊: Investigative Ophthalmology and Visual Science 48・2
• 作者: Matsuda;A;Ebihara;N;Kumagai;N;Fukuda;K;Ebe;K;Hirano;K;Sotozono;C;Tei;M;Hasegawa;K;Shimizu;M;Tamari;M;Namba;K;Ohno;S;Mizuki;N;Ikezawa;Z;Shirakawa;T;Hamuro;J;Kinoshita;S;Matsuda A.
• 通讯作者: Matsuda A.

Genetic polymorphisms in the promoter of the interferon gamma receptor 1 gene are associated with atopic cataracts

• DOI: 10.1167/iovs.06-0991
• 发表时间: 2007-02-01
• 期刊: INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
• 影响因子: 4.4
• 作者: Matsuda, Akira;Ebihara, Nobuyuki;Kinoshita, Shigeru
• 通讯作者: Kinoshita, Shigeru

High-affinity IgE receptor -β chain expression in human mast cells.

人类肥大细胞中高亲和力 IgE 受体 -β 链的表达。

• 发表时间: 2008
• 期刊: J. Immunol. Methods. 336
• 作者: Matsuda A;et al.
• 通讯作者: et al.