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Functions and species differences of softpalate taste buds in mammals

哺乳动物软腭味蕾的功能和物种差异

基本信息

批准号：
18592041
负责人：
HARADA Shuitsu
金额：
$ 2.27万
依托单位：
Kagoshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18592041/
关键词：
soft palate greater superficial petrosal nerve chorda tvmpani nerve taste bud gustatory sensation development basal cell mammal マーカー分子マウス

项目摘要

Gustducin was expressed in almost all (96.7%) IP3R3-expressing cells in taste buds of the soft palate (SP),whereas gustducin-positive cells were 42.4% and 60.1 % of IP3R3-expressing cells in the fungiform (FF) and circumvalate (CV) papillae, respectively. To confirm the broad role of gustducin in the taste transduction on the SP, responses from three major gustatory nerves in gustducin-KO mice were recorded electrophysiologically and the response properties were compared among the nerves. In consistent with the immunohistochemical results in the rat, nerve responses to both sweet and bitter stimuli were markedly reduced in the greater superficial petrosal nerve (GSP) of gustducin-KO mice. In contrast to the GSP, the chorda tympani nerve (CT) and the glossopharngeal nerve (GL) showed reduced responses to sweet and bitter stimuli, respectively. Immunohistochemistry of gustducin and IP3R3 in mice showed that 91.1% of IP3R3-expressing cells in the SP was gustducin positive. These results d … More emonstrate that gustducin is involved in the different lines of the taste-signaling pathway depending on the taste cell differentiation.To examine the embryonic development of the basal cells, the expression of the basal cell markers of taste buds (Shh, Proxi and Mashi) was determined in the mouse embryo by in situ hybridization and immunohistochemistry. Proxi was co-expressed with Shh from the beginning of Shh expression in a punctate pattern on the anterior tongue (E1 2.5) and soft palatal region (E14.5),suggesting that the basal cells of taste buds and Shh-expressing spots in embryos share common features. Mashi expression lagged behind the expression of Shh and Proxi by approximately 2 days in both regions. Nerves reached the epithelium expressing Shh slightly before the onset of Mashi expression. These results suggest that the differentiation of the basal cells in the taste bud starts nerve-independently.We analyzed regenerated taste buds induced by cross-regeneration of CT and GSP in rats and mice using immunohistochemistry and in situ hybridization. The results showed that the gustducin and IP3R3 co-expression between in the SP and FF after the regeneration with the different nerve supply were not depend on the nerve but on the epithelium. This result suggests that the same functional difference still remain after the cross-regeneration. Less

味导素在软腭（SP）味蕾中几乎所有（96.7%）IP3R3表达细胞中表达，而味导素阳性细胞分别占菌状（FF）和环状（CV）乳头中IP3R3表达细胞的42.4%和60.1%。为了证实味导素在 SP 味觉转导中的广泛作用，通过电生理学记录了味导素-KO 小鼠三个主要味觉神经的反应，并比较了神经之间的反应特性。与大鼠的免疫组织化学结果一致，gustducin-KO 小鼠的岩大浅神经 (GSP) 对甜味和苦味刺激的神经反应均显着降低。与 GSP 相比，鼓索神经 (CT) 和舌咽神经 (GL) 分别对甜味和苦味刺激的反应减弱。小鼠中 gustducin 和 IP3R3 的免疫组织化学显示，SP 中 91.1% 表达 IP3R3 的细胞呈 gustducin 阳性。这些结果表明味觉素根据味觉细胞分化参与不同的味觉信号通路。为了检查基底细胞的胚胎发育，通过原位杂交和免疫组织化学测定了小鼠胚胎中味蕾基底细胞标记物（Shh、Proxi 和 Mashi）的表达。 Proxi从Shh表达开始就与Shh共表达，在前舌（E1 2.5）和软腭区（E14.5）呈点状表达，表明味蕾基底细胞和胚胎中Shh表达点具有共同特征。在两个区域中，Mashi 的表达均落后于 Shh 和 Proxi 的表达约 2 天。在 Mashi 表达开始之前，神经到达表达 Shh 的上皮细胞。这些结果表明味蕾基底细胞的分化开始与神经无关。我们利用免疫组织化学和原位杂交分析了大鼠和小鼠中CT和GSP交叉再生诱导的再生味蕾。结果表明，不同神经供应的再生后SP和FF中gustducin和IP3R3的共表达不依赖于神经，而是依赖于上皮。这一结果表明，交叉再生后仍然存在相同的功能差异。较少的

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Expression of Shh,Prox1 and Mash1 during basal cell differentiation of taste buds in the anterior tongue and soft palate of the mouse

小鼠舌前部和软腭味蕾基底细胞分化过程中Shh、Prox1和Mash1的表达

DOI：
发表时间：
2008
期刊：
J Comp Neurol 509(in press)
影响因子：
0
作者：
Nakayama A;Miura H;Shindo Y;Kusakabe Y;Hiroshi T and Harada S
通讯作者：
Hiroshi T and Harada S

Differentiation of the basal cells of mouse taste buds.

小鼠味蕾基底细胞的分化。

DOI：
发表时间：
2006
期刊：
第4回国際シンポジウム「味覚・嗅覚の分子神経機構」招待講演 (招待講演)
影响因子：
0
作者：
Tsukuba;T;Ikeda;S;Okamoto;K;Yasuda;Y;Sakai;E;Kadowaki;T;Sakai;H;Yamamoto;K;三浦祐仁
通讯作者：
三浦祐仁

Expressio of the marker genes for the basal cells of taste buds during mouse embryogenesis

小鼠胚胎发生过程中味蕾基底细胞标记基因的表达