Drug sensitivity and malignant transformation in oral lichen planus using gene expression profiles

目的：探讨应用基因表达谱对口腔扁平苔藓药物敏感性及恶性转化进行基因诊断的可能性。方法：本研究包括6例口腔扁平苔藓患者，他们接受了粘膜活检，证实了他们的疾病。病变部位均为颊黏膜。临床表现为网状型4例，糜烂型2例。采用cDNA阵列分析方法，对6例口腔扁平苔藓中近16600个基因的表达情况进行了分析。我们提取了具有药物敏感性的表达上调或下调的基因。局部使用皮质类固醇治疗他们的病例至少四周。结果：药物有反应者3例，无反应者3例。在近16600个基因中，55个基因的表达水平较高，63个基因的表达水平较低。但在口腔扁平苔藓中未发现有显著表达的基因。应答者表达最低的是PSORS1C2，与牛皮癣有关。结论：经cDNA阵列分析，口腔扁平苔藓中未发现与药物敏感性相关的基因表达。

Objective: To evaluate the possibility of genetic diagnosis on drug sensitivity and malignant transformation in oral lichen planus by gene expression profiles.Methods: Six patients with oral lichen planus were included in this study, who underwent mucosal biopsies that confirmed their disease. The location of the all lesions was buccal mucosa. Clinical forms were reticular form in 4 cases and erosive form in 2 cases. We surveyed the expression of almost 16,600 genes in the 6 cases of oral lichen planus by cDNA array analysis. We extracted gene up-regulated or down-regulated in their expression with drug sensitivity. Topical corticosteroids were used in the treatment of their cases for at least four weeks.Results: Drug responders and non-responders were three cases, respectively. Out of almost 16,600 genes, expression levels of 55 genes with higher and those of 63 genes were lower in drug responders cases. But, there were not any genes to be identified significantly expressed in oral lichen planus. The lowest expression of responders was PSORS1C2, which was related to psoriasis.Conclusions: There were not significantly expressed genes in association with drug sensitivity in oral lichen planus by cDNA array analysis.