Drug sensitivity and malignant transformation in oral lichen planus using gene expression profiles

使用基因表达谱研究口腔扁平苔藓的药物敏感性和恶性转化

• 批准号: 18592163
• 负责人: YAMAZAKI Yutaka
• 金额: $ 2.43万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18592163/
• 关键词: oral lichen planus; drug sensitivity; malignant transformation; microarray; gene expression; 口腔扁苔癬

Objective: To evaluate the possibility of genetic diagnosis on drug sensitivity and malignant transformation in oral lichen planus by gene expression profiles.Methods: Six patients with oral lichen planus were included in this study, who underwent mucosal biopsies that confirmed their disease. The location of the all lesions was buccal mucosa. Clinical forms were reticular form in 4 cases and erosive form in 2 cases. We surveyed the expression of almost 16,600 genes in the 6 cases of oral lichen planus by cDNA array analysis. We extracted gene up-regulated or down-regulated in their expression with drug sensitivity. Topical corticosteroids were used in the treatment of their cases for at least four weeks.Results: Drug responders and non-responders were three cases, respectively. Out of almost 16,600 genes, expression levels of 55 genes with higher and those of 63 genes were lower in drug responders cases. But, there were not any genes to be identified significantly expressed in oral lichen planus. The lowest expression of responders was PSORS1C2, which was related to psoriasis.Conclusions: There were not significantly expressed genes in association with drug sensitivity in oral lichen planus by cDNA array analysis.

目的：探讨基因表达谱在口腔扁平苔藓药敏和恶变基因诊断中的可行性。方法：对6例口腔扁平苔藓患者进行口腔黏膜活检。病变部位均为颊粘膜。临床表现为网状4例，糜烂2例。应用基因芯片技术对6例口腔扁平苔藓组织中近16,600个基因的表达进行了检测。我们提取了对药物敏感的表达上调或下调的基因。使用局部皮质类固醇治疗至少四周。结果：药物应答者和无应答者分别为3例。在近16,600个基因中，有55个基因的表达水平较高，63个基因的表达水平较低。但是，没有发现在口腔扁平苔藓中有显著表达的基因。与银屑病相关的PSORS1C2基因表达水平最低。结论：通过基因芯片分析，口腔扁平苔藓中未发现与药物敏感相关的基因。

项目成果

Expression profiles to predict mode of invasion and lymph-node metastasis of oral squamous cell carcinoma

预测口腔鳞状细胞癌侵袭和淋巴结转移模式的表达谱

• 发表时间: 2007
• 作者: 田沼裕志;松井桂子;川井忠;松井有恒;飯淵信也;佐々木和夫;鈴木治;鎌倉慎治;越後成志;君塚 哲;Kondoh Nobuo;Kondoh Nobuo
• 通讯作者: Kondoh Nobuo