Vascular endothelial growth factor and its receptor to diagnose lymph node metastasis in patients with oral cancer

血管内皮生长因子及其受体诊断口腔癌淋巴结转移

• 批准号: 16591977
• 负责人: YAMAZAKI Yutaka
• 金额: $ 2.05万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16591977/
• 关键词: oral cancer; lymph node metastasis; VEGF; angiogenesis; lymphangiogenesis; serum; 転移

Objective. To evaluate the clinical usefulness of vascular endothelial growth factor (VEGF) family in patients with oral squamous cell carcinoma (OSCC).Methods. Preoperative serum values of VEGF family (VEGF-A,-C, and -D) and their receptor (sVEGF-R1,sVEGF-R2) were measured by enzyme-linked immunosorbent assay in 129 patients with fresh primary oral SCCs. The relationship among VEGF family, clinicopathological parameters, response to therapy and prognosis was investigated.Results. Serum VEGF-A,-C, and -D levels were significantly higher in patients with OSCC than in the healthy control, but both sVEGF-R1 and sVFGF-R2 were not. On the basis of comparision to the 95% range of distribution of serum VEGF family titers in healthy control subjects, the positivity rates of VEGF-A,-C and -D were 41, 31, and 57%, respectively. The serum level of VEGF-A correlated significantly with sex, tumor size and Stage. VEGF-C concentration correlated significantly with distant metastasis, but not with lymph node metastasis. The VEGF-C positive group showed a significantly lower 5-year survival rate than the VEGF-C negative group (63.2% vs. 83.5%, p<0.05). The level of VEGF-D negative correlated with lymph node metastasis and mode of invasion. Moreover, we examined expression of VEGF-C,Flt-4,CD34,CD31 and D2-40 for 24 gingival cancers by immunohistochemistry. Both expressions of VEGF-C and Flt-4 did not correlated with lymph node metastasis. There was no relationship between immunohistochemical and serological methods.Conclusion. The serum level of VEGF-C might have potential usefulness as biological marker of OSCC, but it is necessary to confirm relationship between immunohistochemical and serological methods by a larger scale study

Objective.目的探讨血管内皮生长因子（VEGF）家族在口腔鳞状细胞癌（OSCC）中的临床意义。采用酶联免疫吸附法（ELISA）检测129例口腔鳞癌患者术前血清VEGF家族（VEGF-A、-C、-D）及其受体（sVEGF-R1、sVEGF-R2）水平。探讨VEGF家族与临床病理参数、治疗反应及预后的关系。OSCC患者血清VEGF-A、-C和-D水平显著高于健康对照组，但sVEGF-R1和sVFGF-R2水平均无显著差异。与健康对照组血清VEGF家族滴度的95%分布范围比较，VEGF-A、-C和-D的阳性率分别为41%、31%和57%。血清VEGF-A水平与性别、肿瘤大小、临床分期有关。VEGF-C浓度与远处转移显著相关，与淋巴结转移无关。VEGF-C阳性组的5年生存率明显低于VEGF-C阴性组（63.2%vs.83.5%，p<0.05）。VEGF-D水平与淋巴结转移、浸润方式呈负相关。另外，我们还检测了24例牙龈癌组织中VEGF-C、Flt-4、CD 34、CD 31和D2-40的表达。VEGF-C和Flt-4的表达与淋巴结转移无关。免疫组化与血清学方法无相关性。血清VEGF-C水平可能作为口腔鳞癌的生物学标志物，但免疫组化和血清学方法之间的关系还需更大规模的研究