Genetic diagnosis of micro-metastasis in oral cancer

口腔癌微转移的基因诊断

• 批准号: 12671918
• 负责人: YAMAZAKI Yutaka
• 金额: $ 1.73万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671918/
• 关键词: oral cancer; lymph node metastasis; micrometastasis; p53; MASA; genetic diagnosis; P53

Cervical lymph node metastasis is one of the major prognostic factors in oral squamous cell carcinomas (SCCs). Accurate evaluation of cervical lymph node metastasis is,. therefore, expected to improve the survival rate. It was reported that mutant-allele-specific amplification (MASA) was a sensitive method for detecting micrometastasis in historically negative lymph nodes. In colorectal cancer, it has been shown that genetic diagnosis of lymph node metastasis using MASA was useful for estimating its prognosis. This new approach appears very promising; however, studies in oral SCCs have not been done extensively. To examine the clinical importance of detecting micrometastasis, we screened 235 dissected lymph nodes from ten p53 mutation-positive tumors by MASA to detect micrometastasis. P53 mutations were detected in all histologically metastasis-positive lymph nodes. On the other hand, half of histologically metastasis-negative lymph node have p53 mutations. In these cases, lung metastasis and neck recurrence had occurred in one case, respectively. These data suggest that MASA is useful for detecting cervical lymph node micrometastasis, which is one of important prognostic factors, in oral SCCs.

宫颈淋巴结转移是影响口腔鳞状细胞癌预后的主要因素之一。颈淋巴结转移的准确判断是：因此，有望提高成活率。据报道，突变等位基因特异性扩增（MASA）是检测历史阴性淋巴结微转移的灵敏方法。在结直肠癌中，已经证明使用MASA对淋巴结转移的遗传诊断有助于估计其预后。这种新方法看起来很有希望；然而，口服SCCs的研究尚未广泛开展。为了检验检测微转移的临床意义，我们用MASA方法筛选了10例p53突变阳性肿瘤的235个淋巴结进行微转移检测。在所有组织学转移阳性淋巴结中均检测到P53突变。另一方面，一半的组织学转移阴性淋巴结有p53突变。在这些病例中，肺转移和颈部复发分别发生1例。这些数据表明，MASA对于检测颈部淋巴结微转移是有用的，这是口腔SCCs的重要预后因素之一。

项目成果

山崎 裕: "口腔扁平上皮癌におけるp53遺伝子変異の特徴"日本口腔外科学会雑誌. 47・7. 389-399 (2001)

Yutaka Yamazaki：“口腔鳞状细胞癌中p53基因突变的特征”日本口腔颌面外科杂志47・7（2001年）。

Yamazaki, Y.: "Application of mutant allele specific amplification (MASA) for detecting H-ras mutations during DMBA-induced hamster cheek pouch carcinogenesis"Japanese Journal of Oral and Maxilofacial Surgery. 44. 925-930 (1998)

Yamazaki, Y.：“应用突变等位基因特异性扩增 (MASA) 检测 DMBA 诱导的仓鼠颊囊癌过程中的 H-ras 突变”日本口腔颌面外科杂志。

山崎 裕: "口腔扁平上皮癌におけるp53遺伝子変異の特徴"日本口腔外科学会雑誌. 47. 389-399 (2001)

Yutaka Yamazaki：“口腔鳞状细胞癌中p53基因突变的特征”日本口腔颌面外科杂志47. 389-399（2001）。

Yamazaki, Y.: "Specific regions (L2,L3,LSH) of p53 mutations as prognostic factors in oral squamous cell carcinoma"Japanese Journal of Oral and Maxilofacial Surgery. 47. 649-655 (2001)

Yamazaki, Y.：“p53 突变的特定区域（L2、L3、LSH）作为口腔鳞状细胞癌的预后因素”日本口腔颌面外科杂志。

Yamazaki, Y.: "Characteristics of p53 mutational spectrum in oral squamous cell carcinoma"Japanese Journal of Oral and Maxilofacial Surgery. 47. 389-399 (2001)

Yamazaki, Y.：“口腔鳞状细胞癌中 p53 突变谱的特征”日本口腔颌面外科杂志。