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Identification of Streptoooocus gordoniigenes involved in recruiting Porphyromonas gingivatiscens into a multispecies biofilm

鉴定参与将牙龈卟啉单胞菌招募到多物种生物膜中的戈登链球菌

基本信息

批准号：
18592276
负责人：
KUBONIWA Masae
金额：
$ 2.39万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

项目摘要

Dental plaque biofilm formation proceeds through a developmental pathway initiated by the attachment of pioneer organisms, such as S. gordonii, to tooth surfaces. Through a variety of synergistic interactions, pioneer organisms facilitate the colonization of later arrivals including P. gingivalis, a potential periodontal pathogen. We have investigated genes of S. gordonii required to support a heterotypic biofilm- community with P gingivalis. By screening a plasmid integration library of S gordonii, genes were identified that are crucial for the accumulation of planktonic R gingivalis cells into a multispecies biofilm. These genes were further investigated by specific mutation and complementation analyses. The biofilm-associated genes can be grouped into broad categories based on putative function as follows: i) intercellular or intracellular signaling (cbe and spxB), ii) cell wall integrity and maintenance of adhesive proteins (murE, msrA and atf), iii) extracellular capsule biosynthesis (pgsA and atf), iv) physiology (gdhA, ccmA and ntpB). In addition, a gene for a hypothetical protein was identified. Biofilm visualization and quantitation by confocal microscopy confirmed the role of these genes in the maturation of the multispecies community, including biofilm architectural development. The results suggest that S. gordonii governs the development of heterotypic oral biofilms through multiple genetic pathways.

牙菌斑生物膜的形成是通过一条发育途径进行的，这条途径是由先锋生物体（如沙门氏菌）的附着启动的。gordonii，牙齿表面。通过各种协同相互作用，先锋生物促进了后来者的定植，包括牙龈卟啉单胞菌，一种潜在的牙周病原体。我们研究了S. gordonii所需的支持异型生物膜社区与P牙龈。通过筛选戈登链球菌的质粒整合文库，确定了对嗜酸性牙龈卟啉单胞菌细胞积累成多物种生物膜至关重要的基因。通过特异性突变和互补分析进一步研究这些基因。生物膜相关基因可以基于推定的功能分为如下大类：i）细胞间或细胞内信号传导（cbe和spxB），ii）细胞壁完整性和粘附蛋白的维持（murE、msrA和atf），iii）细胞外囊生物合成（pgsA和atf），iv）生理学（gdhA、ccmA和ntpB）。此外，还鉴定了一种假设蛋白质的基因。生物膜可视化和定量共聚焦显微镜证实了这些基因的作用，在成熟的多物种社区，包括生物膜建筑的发展。结果表明，S.戈登氏菌通过多种遗传途径控制异型口腔生物膜的形成。