Proteomics of Porphyromonas gingivalis within a model oral microbial community

口腔微生物群落模型中牙龈卟啉单胞菌的蛋白质组学

• 批准号: 20592453
• 负责人: KUBONIWA Masae
• 金额: $ 2.75万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20592453/
• 关键词: バイオフイルム; プロテオミクス; 歯周病原性細菌; 代謝経路; ビタミンB; 歯周病; プロテオーム; porphyromonas gingivalis; Fusobacterium nucleatum; Streptococcus gordonii; Porphyromonas gingivalis

P.gingivalis can assemble into a heterotypic community with a middle colonizer F.nucleatum and an early colonizer S.gordonii, and that a community lifestyle provides physiologic support for P.gingivalis. 1156 P.gingivalis proteins, including 403 down-regulated and 89 up-regulated proteins, were detected qualitatively during comparison of the three species model community with P.gingivalis incubated alone under the same conditions. Significant decreases were seen in proteins involved in thiamine, cobalamin, and pyrimidine synthesis. These results indicate that a community lifestyle provides physiologic support for P.gingivalis.

牙龈卟啉单胞菌可以组装成一个异型社区与中间殖民者F.nucleatum和早期殖民者S.gordonii，和社区生活方式提供的生理支持牙龈卟啉单胞菌。结果表明，在相同条件下，牙龈卟啉单胞菌与三种模型菌共培养1156个蛋白质，其中下调蛋白403个，上调蛋白89个。与硫胺素、钴胺素和嘧啶合成有关的蛋白质显著减少。这些结果表明，社区的生活方式提供了牙龈卟啉单胞菌的生理支持。

项目成果

Chapter "Heterotypic Streptococcus gordonii-Porphyromonas gingivalis communities : formation, gene regulation and development", in Genomic Inquiries Into Oral Bacterial Communities (ed. Paul E. Kolenbrander)

“异型戈登链球菌-牙龈卟啉单胞菌群落：形成、基因调控和发育”一章，《口腔细菌群落基因组调查》（Paul E. Kolenbrander 编）

• 作者: Kuboniwa;M.
• 通讯作者: M.

Streptococcus oralis GAPDH peptide inhibits Porphyromonas gingivalis biofilm formation

口腔链球菌 GAPDH 肽抑制牙龈卟啉单胞菌生物膜形成

• 发表时间: 2009
• 作者: Nagata;H.;Iwasaki;M.;Kuboniwa;M.;Maeda;K.;Sekine;S.;Shizukuishi;S.
• 通讯作者: S.

Effect of eucalyptus extract chewing gum on periodontal health: A double-masked, randomized trial

• DOI: 10.1902/jop.2008.070622
• 发表时间: 2008-08-01
• 期刊: JOURNAL OF PERIODONTOLOGY
• 影响因子: 4.3
• 作者: Nagata, Hideki;Inagaki, Yoshika;Shizukuishi, Satoshi
• 通讯作者: Shizukuishi, Satoshi

Distinct roles of long/short fimbriae and gingipains in homotypic biofilm development by Porphyromonas gingivalis.

• DOI: 10.1186/1471-2180-9-105
• 发表时间: 2009-05-26
• 期刊: BMC microbiology
• 影响因子: 4.2
• 作者: Kuboniwa M;Amano A;Hashino E;Yamamoto Y;Inaba H;Hamada N;Nakayama K;Tribble GD;Lamont RJ;Shizukuishi S
• 通讯作者: Shizukuishi S

Subgingival biofilm formation.

• DOI: 10.1111/j.1600-0757.2009.00311.x
• 发表时间: 2010-02
• 期刊: Periodontology 2000
• 影响因子: 18.6
• 作者: Kuboniwa M;Lamont RJ
• 通讯作者: Lamont RJ