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The development for disrase model with periodontitis induced by Cytolethal Distending lbxin derived periodontal pathogens, and application of the model to periodontitis prevention

细胞致死扩张性lbxin源性牙周病原菌诱导的牙周炎模型的建立及其在牙周炎预防中的应用

基本信息

批准号：
18592285
负责人：
AKIFUSA Sumio
金额：
$ 2.32万
依托单位：
Kyushu University (2007)Kyushu Dental College (2006)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18592285/
关键词：
periodontitis Actinobacillus actinomvicetemcomitans Cvtolethal distending toxin sonoporation

项目摘要

It is implied that major cause of tooth loss would be periodontitis in Japanese adolescents. Study on the relationship between oral condition and systemic condition in elderly people revealed that the lowest number of chewable foods were associated with higher risk of mortality than those with the ability to chew all of the 15 types of food surveyed [hazard ratio (BR) = 2.38, 95% confidence interval (95% CI) = 1.07-5.29], though other parameters including current smoking, low serum albumin, and poor physical health status were more significant.Further, reduced chewing ability of soft foods increased the risk (HR = 2.65, 95% CI = 1.20-5.87). Thses evidences suggested that chewing ability was associated with mortality in elderly and may be a predictor for survival rate. Recently, ultrasound-targeting microbubble destruction has been employed in molecular gene therapy, and a new potent nonviral gene transfer method known as 'sonoporation' has been developed. We investigated the efficiency of sonoporation toward growth inhibition of human gingival squamous carcinoma cell line, Ca9-22, in vitro and in vivo. We found that the delivery of BLM by sonoporation induced cytotoxic effect toward Ca9-22 cells in vitro. Our in vivo results showed that tumors nearly disappeared in Ca9-22 cell-implanted nude KSN/slc mice treated with a low dose of BLM followed by sonoporation during the 4-week experimental period. We previously reported that the cytolethal distending toxin B(cdtB)from Actinobacallus actinomycetemcnmitans a periodontopathic bacterium, is responsible for cell cycle arrest and apoptosis in vitro. Thus, we used sonoporation to transfect a cdtB-expressing plasmid into Ca9-22 cells and examined cell viability in vitro and in vivo. We found that an administration of cdtB-expressing plasmid followed by sonoporation-induced marked growth inhibition of Ca9-22 cells and apoptotic cells were also observed in vitro and in vivo.

提示日本青少年牙齿脱落的主要原因是牙周炎。对老年人口腔状况与全身状况关系的研究表明，在调查的15种食物中，咀嚼食物数量最少的老年人的死亡风险高于能够咀嚼所有食物的老年人[风险比（hazard ratio， BR） = 2.38, 95%可信区间(95% CI) = 1.07-5.29]，但吸烟史、低血清白蛋白和身体健康状况不佳等其他参数更为显著。此外，软食物咀嚼能力的降低增加了风险（HR = 2.65, 95% CI = 1.20-5.87）。这些证据表明，咀嚼能力与老年人的死亡率有关，可能是生存率的一个预测指标。近年来，超声靶向微泡破坏已被应用于分子基因治疗中，并开发了一种新的有效的非病毒基因转移方法——“超声穿孔”。我们在体外和体内研究了超声波对人牙龈鳞癌细胞株Ca9-22生长的抑制作用。我们发现通过声孔传递BLM对Ca9-22细胞具有细胞毒性作用。我们的体内实验结果显示，在4周的实验期间，低剂量BLM后超声处理的Ca9-22细胞移植裸KSN/slc小鼠肿瘤几乎消失。我们之前报道了来自牙周病细菌放线菌的细胞致死膨胀毒素B（cdtB）在体外负责细胞周期阻滞和凋亡。因此，我们采用超声法将表达cdtb的质粒转染到Ca9-22细胞中，并在体外和体内检测细胞活力。我们发现，在体外和体内观察到表达cdtb的质粒后，超声刺激诱导的Ca9-22细胞和凋亡细胞的生长明显抑制。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Association Between Body Mass Index and Mortality in an 80‐Year‐Old Population

DOI：
10.1111/j.1532-5415.2007.01170.x
发表时间：
2007-06
期刊：
Journal of the American Geriatrics Society
影响因子：
6.3
作者：
Y. Takata;T. Ansai;I. Soh;S. Akifusa;K. Sonoki;K. Fujisawa;S. Awano;S. Kagiyama;T. Hamasaki;Ikuo Nakamichi;A. Yoshida;T. Takehara
通讯作者：
Y. Takata;T. Ansai;I. Soh;S. Akifusa;K. Sonoki;K. Fujisawa;S. Awano;S. Kagiyama;T. Hamasaki;Ikuo Nakamichi;A. Yoshida;T. Takehara

Physical fitness and 4-year mortality in an 80-year-old population