Real-time imaging of stress-related receptor dynamics in the brain

本研究的最终目的是阐明糖皮质激素受体（GR）和盐皮质激素受体（MR）在大脑中如何响应激素环境波动在应激反应和昼夜节律直接可视化这些受体作为一个整体的大脑水平。作为初步研究，我们使用从GFP-GR基因敲入小鼠获得的原代培养神经元分析GR的动力学，此外，我们使用双光子激光显微镜从全脑水平观察来自GFP-GR基因敲入小鼠的固定脑。我们成功地在GFP-GR基因敲入小鼠大脑皮层第二层的深度直接显示了GFP-GR。

The ultimate aim of the present study is to clarify how glucocorticoid receptor (GR) and mineralocorticoid receptor (MR) in the brain respond to hormonal milieu fluctuated during stress response and circadian rhythm by directly visualizing these receptors as a whole brain level. As a preliminary study, we analyzed dynamics of GR using primary cultured neurons obtained from GFP-GR knock-in mice, and furthermore, we observed a fixed brain from GFP-GR knock-in mouse as a whole brain level using a two-photon laser microscope. We succeeded a direct visualization of GFP-GR at the depth of layer II of cerebral cortex of GFP-GR knock-in mouse.