Development of diagnostic methods for Parkinson disease using high fieled magnetic resonance imaging

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We examined the feasibility of high filed MRI, particularly ^<19>F-MRI, for the diagnosis of Parkinson disease using PC12 cells and 6OH-dopamine treated rats. We measured ^<19>F-NMR signals in the homogenates of rat brain and several agents such as 6-^<19>F-fluoro-L-DOPA (^<19>F-DOPA) and chemicals that are involved in the activity of dopamine neurons. Among them, ^<19>F-DOPA showed the best signal to nose ratio. When ^<19>F-DOPA was added into culture medium of PC12 cells, MR images of ^<19>F-DOPA in about 50 mg of PC12 cells were successfully detected. When we measured the brain from 6-OH-dopamine treated rats that received intra-peritoneal injection of ^<19>F-DOPA, MR images of ^<19>F-DOPA in the striatum were successfully detected by 10 hours measurements of 7-tesla MRI. This study gives us the fundamental data for the development of diagnostic methods for Parkinson disease using high fieled MRI.